Honda T, Arita M, Miwatani T
Infect Immun. 1984 Mar;43(3):959-65. doi: 10.1128/iai.43.3.959-965.1984.
Enterotoxigenic Escherichia coli strains were divided into five groups on the basis of their bacterial surface hydrophobicity (Honda et al., FEMS Microbiol. Lett. 17:273-276, 1983). Strains in group III showed heat-stable high hydrophobicity, although they did not show mannose-resistant hemagglutination with either human or bovine erythrocytes. E. coli strain 260-1 in group III was characterized. Electron microscopic examination revealed the presence of pili on the surface of this strain, but not on that of strain 260-1a, which is a mutant of 260-1 showing low hydrophobicity. When strain 260-1 was grown at 18 degrees C, it did not produce pili or show high hydrophobicity. On homogenization of strain 260-1 grown at 37 degrees C the high hydrophobicity and the pili on its surface were lost. These results indicate that the pili of strain 260-1 are associated with the hydrophobicity. Strain 260-1 pili were purified to homogeneity by successive column chromatographies on Sepharose 4B and phenyl-Sepharose CL-4B. Their molecular weight was estimated to be about 18,000. An antigenic difference between purified pili of strain 260-1 and colonization factor antigens I and II was demonstrated. The colonization ability of E. coli 260-1 was shown by animal experiments on suckling mice and infant rabbits. From these results it is concluded that the pili of strains in group III of human enterotoxigenic E. coli, which may play a role in colonization, are of a new type.
产肠毒素大肠杆菌菌株根据其细菌表面疏水性分为五组（本田等人，《FEMS微生物学快报》17:273 - 276，1983年）。第三组菌株表现出热稳定的高疏水性，尽管它们与人或牛红细胞均不表现出甘露糖抗性血凝反应。对第三组中的大肠杆菌菌株260 - 1进行了特性分析。电子显微镜检查显示该菌株表面存在菌毛，但疏水性低的260 - 1a菌株（260 - 1的突变体）表面没有菌毛。当菌株260 - 1在18℃下生长时，它不产生菌毛也不表现出高疏水性。将在37℃下生长的菌株260 - 1匀浆后，其表面的高疏水性和菌毛丧失。这些结果表明菌株260 - 1的菌毛与疏水性有关。通过在琼脂糖4B和苯基琼脂糖CL - 4B上连续柱层析将菌株260 - 1的菌毛纯化至均一。其分子量估计约为18,000。已证明菌株260 - 1纯化菌毛与定居因子抗原I和II之间存在抗原差异。通过对乳鼠和幼兔的动物实验显示了大肠杆菌260 - 1的定居能力。从这些结果可以得出结论，人产肠毒素大肠杆菌第三组菌株的菌毛可能在定居中起作用，是一种新型菌毛。
