通过聚合酶链反应检测侵袭（A）和质粒毒力（C）基因对来自后院家禽的鸡白痢沙门氏菌分离株进行鉴定
Characterization of Gallinarum isolates from backyard poultry by polymerase chain reaction detection of invasion (A) and plasmid virulence (C) genes.
作者信息
Pal Susmita, Dey Samir, Batabyal Kunal, Banerjee Abhiroop, Joardar Siddhartha Narayan, Samanta Indranil, Isore Devi Prasad
机构信息
Department of Veterinary Microbiology, West Bengal University of Animal and Fishery Sciences, Belgachia, Kolkata - 700 037, West Bengal, India.
出版信息
Vet World. 2017 Jul;10(7):814-817. doi: 10.14202/vetworld.2017.814-817. Epub 2017 Jul 23.
AIM
The aim was to characterize serovar Gallinarum isolated from backyard poultry by polymerase chain reaction (PCR) detection of virulence genes invasion (A) and plasmid virulence C (C).
MATERIALS AND METHODS
Two strains of serovar Gallinarum isolates used in this study were obtained from an outbreak of fowl typhoid in backyard Vanaraja fowl. PCR technique was used for detection of A and C genes using standard methodology. The A PCR product from one representative isolate was sequenced and compared with other related serovars in GenBank data.
RESULTS
Gallinarum produced expected amplicons of A and C gene products. Nucleotide sequence of 285 bp A gene was deposited in GenBank with accession no. KX788214. Sequence analysis of A gene was found conserved in serovars and demonstrated 100% homology with closely related serovars of .
CONCLUSION
Invasion gene (A) was found to be highly conserved in Gallinarum and highly similar with closely related serovars. The isolates also contained plasmid-mediated spvC gene indicating possession of virulence plasmid.
目的
通过聚合酶链反应（PCR）检测毒力基因侵袭（A）和质粒毒力C（C），对从后院家禽中分离出的鸡白痢沙门氏菌血清型进行特征分析。
材料与方法
本研究中使用的两株鸡白痢沙门氏菌分离株取自后院凡纳拉亚鸡爆发的禽伤寒疫情。采用PCR技术，运用标准方法检测A和C基因。对一株代表性分离株的A基因PCR产物进行测序，并与GenBank数据库中的其他相关血清型进行比较。
结果
鸡白痢沙门氏菌产生了预期的A和C基因产物扩增子。285bp A基因的核苷酸序列已存入GenBank，登录号为KX788214。A基因的序列分析表明，该基因在血清型中保守，与密切相关血清型的同源性为100%。
结论
发现侵袭基因（A）在鸡白痢沙门氏菌中高度保守，且与密切相关血清型高度相似。这些分离株还含有质粒介导的spvC基因，表明其具有毒力质粒。
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