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百日咳毒素S-1催化亚基在大肠杆菌中的表达。

Expression of the S-1 catalytic subunit of pertussis toxin in Escherichia coli.

作者信息

Barbieri J T, Rappuoli R, Collier R J

出版信息

Infect Immun. 1987 May;55(5):1321-3. doi: 10.1128/iai.55.5.1321-1323.1987.

DOI:10.1128/iai.55.5.1321-1323.1987
PMID:2883125
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC260508/
Abstract

The S-1 subunit of pertussis toxin was expressed as a fusion protein in a strain of Escherichia coli deficient in protein degradation. The fusion protein reacted with anti-pertussis toxin antibody, and, like authentic pertussis toxin, it ADP-ribosylated a 41,000-molecular-weight membrane protein from human erythrocytes.

摘要

百日咳毒素的S-1亚基在一株缺乏蛋白质降解能力的大肠杆菌中表达为融合蛋白。该融合蛋白与抗百日咳毒素抗体发生反应，并且与天然百日咳毒素一样，它能使来自人红细胞的一种分子量为41,000的膜蛋白发生ADP核糖基化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b36b/260508/339faac3f15d/iai00089-0312-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b36b/260508/670032f20126/iai00089-0312-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b36b/260508/5526be25b71b/iai00089-0312-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b36b/260508/339faac3f15d/iai00089-0312-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b36b/260508/670032f20126/iai00089-0312-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b36b/260508/5526be25b71b/iai00089-0312-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b36b/260508/339faac3f15d/iai00089-0312-c.jpg

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本文引用的文献

1
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Activation by thiol of the latent NAD glycohydrolase and ADP-ribosyltransferase activities of Bordetella pertussis toxin (islet-activating protein).硫醇对百日咳博德特氏菌毒素（胰岛激活蛋白）潜在的NAD糖水解酶和ADP核糖基转移酶活性的激活作用。
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Tn5-induced mutations affecting virulence factors of Bordetella pertussis.
变形链球菌主要表面蛋白抗原P1（I/II）在大肠杆菌中的分子克隆与表达。
Infect Immun. 1988 Aug;56(8):2114-9. doi: 10.1128/iai.56.8.2114-2119.1988.
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ADP-ribosyltransferase mutations in the catalytic S-1 subunit of pertussis toxin.百日咳毒素催化性S-1亚基中的ADP-核糖基转移酶突变
Infect Immun. 1988 Aug;56(8):1934-41. doi: 10.1128/iai.56.8.1934-1941.1988.
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Construction and characterization of Bordetella pertussis toxin mutants.百日咳博德特氏菌毒素突变体的构建与特性分析
Infect Immun. 1987 Oct;55(10):2465-70. doi: 10.1128/iai.55.10.2465-2470.1987.
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Photolabeling of Glu-129 of the S-1 subunit of pertussis toxin with NAD.用烟酰胺腺嘌呤二核苷酸对百日咳毒素S-1亚基的Glu-129进行光标记。
Infect Immun. 1989 Nov;57(11):3549-54. doi: 10.1128/iai.57.11.3549-3554.1989.
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8
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9
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Proc Natl Acad Sci U S A. 1988 Jul;85(13):4667-71. doi: 10.1073/pnas.85.13.4667.
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Photolabelling of mutant forms of the S1 subunit of pertussis toxin with NAD+.用NAD+对百日咳毒素S1亚基的突变形式进行光标记。
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4
Subunit structure of islet-activating protein, pertussis toxin, in conformity with the A-B model.百日咳毒素胰岛激活蛋白的亚基结构，符合A-B模型。
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J Biol Chem. 1982 Apr 10;257(7):3739-46.
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