Singer R, Sagiv M, Bruchis S, Barnet M, Kaufman H, Servadio C
Int J Fertil. 1987 Mar-Apr;32(2):145-8.
Total and free testosterone (T) and estradiol (E2) were determined by a radioimmunoassay technique in 30 normozoospermic and oligozoospermic human semen samples. The mean amounts of total T ranged from 25.1 +/- 7.4 to 32.5 +/- 12.2 ng/100 mL, and those of the free hormone from 7.0 +/- 2.8 to 7.9 +/- 2.3 ng/100 mL. The values for E2 were 10.5 +/- 4.4 to 11.2 +/- 4.5 pg/mL and 2.3 +/- 1.1 to 3.2 +/- 2.6 pg/mL, respectively. There was no correlation between sperm density of semen and the levels of T and E2. It is suggested that the nonspecific binding of T and E2 in semen provides a physiological reservoir for active, free T and E2 and that an appropriate ratio of free T to free E2 in semen may be crucial in the processes that lead to fertilization.
采用放射免疫分析技术测定了30份正常精子数和少精子数的人类精液样本中的总睾酮（T）、游离睾酮以及雌二醇（E2）。总T的平均含量范围为25.1±7.4至32.5±12.2 ng/100 mL，游离激素的平均含量范围为7.0±2.8至7.9±2.3 ng/100 mL。E2的值分别为10.5±4.4至11.2±4.5 pg/mL以及2.3±1.1至3.2±2.6 pg/mL。精液中的精子密度与T和E2水平之间无相关性。提示精液中T和E2的非特异性结合为活性游离T和E2提供了一个生理储备库，并且精液中游离T与游离E2的适当比例在导致受精的过程中可能至关重要。
