Oh Min-Kyun, Yoon Kyung Jae, Lee Yong-Taek, Chae Seoung Wan, Choi Hye Young, Shin Hee Suk, Park Yun Hee, Chun Se-Woong, Park Young Sook
1 Department of Rehabilitation Medicine, Gyeongsang National University Changwon Hospital, Gyeongsang National University School of Medicine, Changwon, Republic of Korea.
2 Department of Physical & Rehabilitation Medicine, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
J Int Med Res. 2018 Jan;46(1):249-257. doi: 10.1177/0300060517723799. Epub 2017 Aug 23.
Objective To evaluate the effects of zolpidem on functional recovery in a rat model of acute ischemic stroke. Methods Following ischemic stroke procedures, 42 rats (six in each group) were randomly assigned to receive zolpidem (0.1, 0.25, 0.5, 1.0, 2.0 or 4.0 mg/kg) or normal saline administer intraperitoneally once daily for two weeks. Motor behavioural index (MBI) scores, radial 8-arm maze (RAM) test times and brain MRI scans were obtained 24 hours (Day 1) and two weeks (Day 14) post-procedure. Immunohistochemistry was performed on Day 14. Results By comparison with the normal saline group, the 0.5 and 1.0 mg/kg zolpidem groups showed statistically significant improvements in MBI scores and increased numbers of brain-derived neurotrophic factor (BDNF) stained cells over the two week dosing period. By contrast, the 4.0 mg/kg zolpidem group had statistically significantly impaired MBI scores compared with the control group. No differences among groups were found in RAM times or infarction volumes. Conclusions This study in a rat model showed that 0.5-1.0 mg/kg of zolpidem had beneficial effects on behavioural recovery by enhancing neural plasticity without causing any memory impairment in acute ischemic stroke.
目的 评估唑吡坦对急性缺血性脑卒中大鼠模型功能恢复的影响。方法 在缺血性脑卒中手术之后，将42只大鼠（每组6只）随机分组，分别腹腔注射唑吡坦（0.1、0.25、0.5、1.0、2.0或4.0mg/kg）或生理盐水，每日一次，持续两周。在手术后24小时（第1天）和两周（第14天）获取运动行为指数（MBI）评分、放射状八臂迷宫（RAM）测试时间及脑部MRI扫描结果。在第14天进行免疫组织化学检测。结果 与生理盐水组相比，在为期两周的给药期内，0.5和1.0mg/kg唑吡坦组的MBI评分有统计学显著改善，脑源性神经营养因子（BDNF）染色细胞数量增加。相比之下，4.0mg/kg唑吡坦组的MBI评分与对照组相比有统计学显著受损。各组在RAM测试时间或梗死体积方面未发现差异。结论 该大鼠模型研究表明，0.5 - 1.0mg/kg的唑吡坦通过增强神经可塑性对行为恢复有有益作用，且在急性缺血性脑卒中中不会引起任何记忆损害。
