Sadullahoğlu Canan, Dere Yelda, Atasever Türkan Rezanko, Öztop Mine Tunakan, Karaaslan Önder
Department of Pathology, Antalya Training and Research Hospital, Antalya, Turkey.
Turk Patoloji Derg. 2017;1(1):223-227. doi: 10.5146/tjpath.2017.01402.
Dermatofibroma (DF) is a benign fibrohistiocytic tumor whereas dermatofibrosarcoma protuberans (DFSP) has intermediate malignant potential. CD34 is the most commonly used antibody in differentiating these tumors. Various studies have stated the rates of D2-40 expression as 0-50% in DFSPs and 86-100% in DFs. Our aim in this study was to determine the expression of CD34 and D2-40 in DFs and DFSPs and the possible use of D2-40 in the differential diagnosis of these lesions.
This is a retrospective study including 30 DF and 15 DFSP cases which were reevaluated for epidermal changes, the presence of a transmission zone (Grenz zone), infiltration of soft tissues, infiltration pattern and histologic subtypes in addition to cellular pleomorphism, nuclear atypia, and necrosis. A manual immunohistochemistry procedure was performed with D2-40 and CD 34 antibodies using a representative paraffin block.
The average age was 37.36 and 42.86 years in the DF and DFSP cases. The average diameter was 0.9 and 5.03 cm, respectively, for the DFs and DFSPs. There was a significant correlation between the two entities for sex, localization and diameter of the lesion. A significant difference was found between the positivity of CD34 and D2-40 in DFs and DFSPs.
Additional immunohistochemical markers may be needed in DFs with CD34 positivity. Our results showed the additional helpful role of this marker in problematic cases.
皮肤纤维瘤（DF）是一种良性纤维组织细胞瘤，而隆突性皮肤纤维肉瘤（DFSP）具有中等恶性潜能。CD34是鉴别这些肿瘤最常用的抗体。各种研究表明，DFSP中D2-40的表达率为0%-50%，DF中为86%-100%。本研究的目的是确定CD34和D2-40在DF和DFSP中的表达情况，以及D2-40在这些病变鉴别诊断中的可能用途。
这是一项回顾性研究，纳入30例DF和15例DFSP病例，除了细胞多形性、核异型性和坏死外，还对表皮变化、移行带（无细胞带）的存在、软组织浸润、浸润模式和组织学亚型进行了重新评估。使用代表性石蜡块，采用手动免疫组织化学方法检测D2-40和CD 34抗体。
DF和DFSP病例的平均年龄分别为37.36岁和42.86岁。DF和DFSP的平均直径分别为0.9 cm和5.03 cm。在病变的性别、部位和直径方面，这两种实体之间存在显著相关性。DF和DFSP中CD34和D2-40的阳性率存在显著差异。
CD34阳性的DF可能需要额外的免疫组织化学标志物。我们的结果显示了该标志物在疑难病例中的额外辅助作用。
