利用用于串行晶体学的室温样品递送方法，通过X射线自由电子激光揭示了来自双吲哚霉素聚酮合酶的酰基转移酶的构象灵活性。
The Conformational Flexibility of the Acyltransferase from the Disorazole Polyketide Synthase Is Revealed by an X-ray Free-Electron Laser Using a Room-Temperature Sample Delivery Method for Serial Crystallography.
作者信息
Mathews Irimpan I, Allison Kim, Robbins Thomas, Lyubimov Artem Y, Uervirojnangkoorn Monarin, Brunger Axel T, Khosla Chaitan, DeMirci Hasan, McPhillips Scott E, Hollenbeck Michael, Soltis Michael, Cohen Aina E
机构信息
Stanford Synchrotron Radiation Lightsource , 2575 Sand Hill Road, Menlo Park, California 94025, United States.
出版信息
Biochemistry. 2017 Sep 12;56(36):4751-4756. doi: 10.1021/acs.biochem.7b00711. Epub 2017 Aug 31.
Abstract
The crystal structure of the trans-acyltransferase (AT) from the disorazole polyketide synthase (PKS) was determined at room temperature to a resolution of 2.5 Å using a new method for the direct delivery of the sample into an X-ray free-electron laser. A novel sample extractor efficiently delivered limited quantities of microcrystals directly from the native crystallization solution into the X-ray beam at room temperature. The AT structure revealed important catalytic features of this core PKS enzyme, including the occurrence of conformational changes around the active site. The implications of these conformational changes for polyketide synthase reaction dynamics are discussed.
摘要
利用一种将样品直接输送到X射线自由电子激光的新方法，在室温下测定了来自双吲哚霉素聚酮合酶（PKS）的反式酰基转移酶（AT）的晶体结构，分辨率为2.5埃。一种新型样品提取器能在室温下将有限数量的微晶从天然结晶溶液中直接有效地输送到X射线束中。AT结构揭示了这种核心PKS酶的重要催化特征，包括活性位点周围构象变化的发生。讨论了这些构象变化对聚酮合酶反应动力学的影响。
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