RIKEN SPring-8 Center, Sayo, Japan.
Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, Japan.
Nat Methods. 2015 Jan;12(1):61-3. doi: 10.1038/nmeth.3172. Epub 2014 Nov 10.
Serial femtosecond X-ray crystallography (SFX) has revolutionized atomic-resolution structural investigation by expanding applicability to micrometer-sized protein crystals, even at room temperature, and by enabling dynamics studies. However, reliable crystal-carrying media for SFX are lacking. Here we introduce a grease-matrix carrier for protein microcrystals and obtain the structures of lysozyme, glucose isomerase, thaumatin and fatty acid-binding protein type 3 under ambient conditions at a resolution of or finer than 2 Å.
连续飞秒 X 射线晶体学（SFX）通过将适用性扩展到微米级蛋白质晶体，甚至在室温下，以及通过实现动力学研究，彻底改变了原子分辨率结构研究。然而，SFX 的可靠晶体载体仍然缺乏。在这里，我们介绍了一种蛋白质微晶体的油脂基质载体，并在环境条件下获得了溶菌酶、葡萄糖异构酶、天花粉和脂肪酸结合蛋白 3 的结构，分辨率达到或优于 2Å。
