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人副流感病毒3型基质蛋白基因的序列分析:副粘病毒之间广泛的序列同源性

Sequence analysis of the matrix protein gene of human parainfluenza virus type 3: extensive sequence homology among paramyxoviruses.

作者信息

Spriggs M K, Johnson P R, Collins P L

出版信息

J Gen Virol. 1987 May;68 ( Pt 5):1491-7. doi: 10.1099/0022-1317-68-5-1491.

DOI:10.1099/0022-1317-68-5-1491
PMID:2883252
Abstract

The sequences of the human parainfluenza virus type 3 (PIV3) matrix (M) mRNA [1150 nucleotides exclusive of poly(A)] and predicted M protein (353 amino acids) were determined by sequence analysis of cloned cDNA and viral genomic RNA. The gene-end sequence of the M gene differed from the semi-conserved gene-end sequence of the other PIV3 genes by an apparent insertion of eight nucleotides. The PIV3 M protein shared high sequence homology with Sendai virus and moderate homology with measles virus and canine distemper virus. Statistical analysis of the available sequences showed that the M protein was the most highly conserved parainfluenza viral protein.

摘要

通过对克隆的互补脱氧核糖核酸(cDNA)和病毒基因组核糖核酸(RNA)进行序列分析,确定了人3型副流感病毒(PIV3)基质(M)信使核糖核酸(mRNA)的序列[1150个核苷酸,不包括聚腺苷酸(polyA)]以及预测的M蛋白(353个氨基酸)。M基因的基因末端序列与其他PIV3基因的半保守基因末端序列不同,明显插入了八个核苷酸。PIV3 M蛋白与仙台病毒具有高度的序列同源性,与麻疹病毒和犬瘟热病毒具有中等同源性。对现有序列的统计分析表明,M蛋白是副流感病毒中保守性最高的蛋白。

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