大鼠脑离散杏仁核中γ-氨基丁酸和谷氨酸的质量碎片分析法测定

Mass fragmentographic determination of gamma-aminobutyric acid and glutamic acid in discrete amygdaloid nuclei of rat brain.

作者信息

Nakamura K, Matsumoto T, Hirano M, Kagoshima H, Kuroki T, Yao H, Uchimura H, Nakahara T

出版信息

J Neurochem. 1987 Jun;48(6):1842-4. doi: 10.1111/j.1471-4159.1987.tb05745.x.

DOI:10.1111/j.1471-4159.1987.tb05745.x

Abstract

A mass fragmentographic method for the simultaneous quantification of gamma-aminobutyric acid (GABA) and glutamic acid is described. In a convenient one-step reaction, the two amino acids were derivatized with pentafluoropropionic anhydride and pentafluoropropanol. The derivatization products were stable for several days. The technique has been applied to the assay of GABA and Glu in five amygdaloid nuclei of the rat brain. The GABA level was high in the central and medial nuclei, whereas the Glu level was high in the lateral and basal nuclei. The regional distribution of GABA was different from that of Glu within the amygdaloid nuclei.

摘要

描述了一种同时定量γ-氨基丁酸（GABA）和谷氨酸的质量碎片分析法。在一个简便的一步反应中，这两种氨基酸用五氟丙酸酐和五氟丙醇进行衍生化。衍生化产物可稳定保存数天。该技术已应用于大鼠脑五个杏仁核中GABA和Glu的测定。中央核和内侧核中的GABA水平较高，而外侧核和基底核中的Glu水平较高。杏仁核内GABA的区域分布与Glu不同。