胰高血糖素对正常甲状腺和甲状腺功能减退大鼠肝细胞中环磷酸腺苷（cAMP）积累及生酮作用的影响。

Effects of glucagon on cAMP accumulation and ketogenesis in hepatocytes from euthyroid and hypothyroid rats.

作者信息

Shaheen O, Otham S, Al-Turk W A, Sliman N

出版信息

J Pharm Pharmacol. 1987 Mar;39(3):169-72. doi: 10.1111/j.2042-7158.1987.tb06243.x.

DOI:10.1111/j.2042-7158.1987.tb06243.x

Abstract

The resistance to the effects of glucagon was studied in isolated hepatocytes prepared from male rats treated with 6N-propyl-2-thiouracil (PTU). Incorporation of [14C]oleate into ketone bodies in response to various concentrations of glucagon (10(-5) to 10(-10) M) was reduced in hepatocytes from hypothyroid rats compared with the euthyroid group. The reduced sensitivity to the effects of glucagon on ketogenesis after treatment with PTU was associated with a reduced ability of those hepatocytes to maintain cyclic adenosine-3',5'-monophosphate (cAMP) at levels required to stimulate ketogenesis. The concentration of cAMP in response to glucagon (10(-5) to 10(-10) M) was diminished in hepatocytes from hypothyroid rats, compared with those from euthyroid animals.

摘要

在由用6-N-丙基-2-硫氧嘧啶（PTU）处理的雄性大鼠制备的分离肝细胞中，研究了对胰高血糖素作用的抵抗性。与甲状腺功能正常组相比，甲状腺功能减退大鼠的肝细胞中，响应于各种浓度胰高血糖素（10^(-5)至10^(-10) M）的[14C]油酸酯掺入酮体的量减少。PTU处理后对胰高血糖素在生酮作用上的敏感性降低，与这些肝细胞将环腺苷-3',5'-单磷酸（cAMP）维持在刺激生酮作用所需水平的能力降低有关。与甲状腺功能正常动物的肝细胞相比，甲状腺功能减退大鼠的肝细胞中响应于胰高血糖素（10^(-5)至10^(-10) M）的cAMP浓度降低。