Shaheen O, Otham S, Al-Turk W A, Sliman N
J Pharm Pharmacol. 1987 Mar;39(3):169-72. doi: 10.1111/j.2042-7158.1987.tb06243.x.
The resistance to the effects of glucagon was studied in isolated hepatocytes prepared from male rats treated with 6N-propyl-2-thiouracil (PTU). Incorporation of [14C]oleate into ketone bodies in response to various concentrations of glucagon (10(-5) to 10(-10) M) was reduced in hepatocytes from hypothyroid rats compared with the euthyroid group. The reduced sensitivity to the effects of glucagon on ketogenesis after treatment with PTU was associated with a reduced ability of those hepatocytes to maintain cyclic adenosine-3',5'-monophosphate (cAMP) at levels required to stimulate ketogenesis. The concentration of cAMP in response to glucagon (10(-5) to 10(-10) M) was diminished in hepatocytes from hypothyroid rats, compared with those from euthyroid animals.
在由用6-N-丙基-2-硫氧嘧啶（PTU）处理的雄性大鼠制备的分离肝细胞中，研究了对胰高血糖素作用的抵抗性。与甲状腺功能正常组相比，甲状腺功能减退大鼠的肝细胞中，响应于各种浓度胰高血糖素（10^(-5)至10^(-10) M）的[14C]油酸酯掺入酮体的量减少。PTU处理后对胰高血糖素在生酮作用上的敏感性降低，与这些肝细胞将环腺苷-3',5'-单磷酸（cAMP）维持在刺激生酮作用所需水平的能力降低有关。与甲状腺功能正常动物的肝细胞相比，甲状腺功能减退大鼠的肝细胞中响应于胰高血糖素（10^(-5)至10^(-10) M）的cAMP浓度降低。
