Parnas I, Parnas H
J Physiol (Paris). 1986;81(4):289-305.
A new Ca-voltage hypothesis for neurotransmitter release is proposed. Accordingly, membrane depolarization has two roles. To increase membrane conductance to calcium and to activate a molecule S from an inactive form T. Only the active form S binds calcium ions to start the chain of events leading to release. Four lines of experiments are described to support this hypothesis. Disassociation between Ca2+ entry and transmitter release. Loading of the terminal with Ca2+ and obtaining release with little additional entry of Ca2+. Measurements of kinetics of release. Modulation of release by changes in membrane potential. Recent criticism as to the validity of the experimental techniques used in the first two lines of experiments is analyzed and rejected.
提出了一种关于神经递质释放的新钙电压假说。据此，膜去极化有两个作用。增加膜对钙的电导，并将分子S从无活性形式T激活。只有活性形式S结合钙离子以启动导致释放的一系列事件。描述了四项实验来支持这一假说。钙离子进入与递质释放之间的解离。用钙离子加载终末并在几乎没有额外钙离子进入的情况下实现释放。释放动力学的测量。通过膜电位变化对释放进行调节。分析并驳回了近期对前两项实验中所用实验技术有效性的批评。
