Banach Justin C, Clark Stephanie, Lamsal Buddhi P
Iowa State University, Food Science and Human Nutrition, Food Sciences Building, Ames, IA, USA.
J Sci Food Agric. 2018 Jan;98(1):391-399. doi: 10.1002/jsfa.8632. Epub 2017 Sep 25.
Extruded and ground milk protein concentrate powders, specifically those with 800 g kg protein (i.e. MPC80), imparted softness, cohesion and textural stability to high-protein nutrition (HPN) bars. The present study evaluated some physicochemical properties of extruded and conventionally produced (i.e. spray-dried) MPC80 to explain these improvements. Protein chemical changes and aggregations within MPC80-formulated HPN bars during storage were characterized.
Extruded MPC80 powders had broader particle size distribution (P < 0.05) and smaller volume-weighted mean diameter (P < 0.05) than the spray-dried control. Loose, tapped and particle densities increased (P < 0.05) and correspondingly occluded and interstitial air volumes decreased (P < 0.05) after extruding and milling MPC80. Extrusion decreased water holding capacity (P < 0.05) and solubility (P < 0.05), yet improved the wettability (P < 0.05) of MPC80. MPC80 free sulfhydryl (P < 0.05) and free amine (P < 0.05) concentrations decreased after extrusion. Sulfhydryl and amine concentrations changed (P < 0.05) and disulfide-linked and, more prominently, Maillard-induced aggregates developed during HPN bar storage.
Extrusion and milling together changed the physicochemical properties of MPC80. Chemical changes and protein aggregations occurred in HPN bars prepared with either type of MPC80. Thus, the physicochemical properties of the formulating powder require consideration for desired HPN bar texture and stability. © 2017 Society of Chemical Industry.
挤压和研磨的乳蛋白浓缩粉,特别是蛋白质含量为800 g/kg的那些(即MPC80),赋予了高蛋白营养(HPN)棒柔软性、粘性和质地稳定性。本研究评估了挤压和传统生产(即喷雾干燥)的MPC80的一些物理化学性质,以解释这些改善。对MPC80配方的HPN棒在储存期间的蛋白质化学变化和聚集情况进行了表征。
与喷雾干燥对照相比,挤压的MPC80粉末具有更宽的粒度分布(P < 0.05)和更小的体积加权平均直径(P < 0.05)。挤压和研磨MPC80后,松装密度、振实密度和颗粒密度增加(P < 0.05),相应地,封闭空气体积和间隙空气体积减少(P < 0.05)。挤压降低了MPC80的持水能力(P < 0.05)和溶解度(P < 0.05),但提高了其润湿性(P < 0.05)。挤压后MPC80的游离巯基(P < 0.05)和游离胺(P < 0.05)浓度降低。在HPN棒储存期间,巯基和胺浓度发生变化(P < 0.05),二硫键连接的聚集体,更显著的是美拉德反应诱导的聚集体形成。
挤压和研磨共同改变了MPC80的物理化学性质。用任何一种MPC80制备的HPN棒中都发生了化学变化和蛋白质聚集。因此,为了获得所需的HPN棒质地和稳定性,需要考虑配方粉末的物理化学性质。© 2017化学工业协会。