Zhang C, Zhu S, Jatt A-N, Pan Y, Zeng M
College of Food Science and Engineering, Ocean University of China, Qingdao, China.
Institute of Microbiology, University of Sindh, Sindh, Pakistan.
Lett Appl Microbiol. 2017 Nov;65(5):388-394. doi: 10.1111/lam.12795. Epub 2017 Oct 10.
Shewanella spp. are the common spoilage organisms found in aquatic food products stored at low temperature and their spoilage mechanism has been reported to be mediated by quorum sensing (QS). However, the specifically expressed proteins responding to N-acyl homoserine-lactone (AHLs) were seldom reported. This study aims to evaluate the effects of different AHL signal molecules on Shewanella putrefaciens Z4 isolated from refrigerated turbot (Scophthalmus maximus) at the proteome level. The results revealed that exogenous AHLs were utilized as QS signal molecules by S. putrefaciens Z4, and AHLs were not degraded by intracellular or extracellular enzymes secreted by S. putrefaciens Z4. Twenty-three differently expressed spots upon the addition of AHLs were selected and identified by liquid chromatography-mass spectrometry (LC-MS). The results indicated that proteins involving in growth and metabolism (i.e. citrate synthase, succinate semialdehyde dehydrogenase), environment adaptation and regulators (i.e. polysaccharide deacetylases, transaldolase) were down-regulated upon three kinds of AHLs (C4-HSL, C6-HSL and O-C6-HSL), whereas the abundance of stress response protein and DNA ligase were elevated by the addition of exogenous AHLs. Moreover, the effects of exogenous C6-HSL and O-C6-HSLwere prominent. These results provide evidence that AHL-based QS signal molecules affected some important metabolic properties of S. putrefaciens.
N-acyl homoserine lactone (AHLs)-based quorum sensing signal molecules involving in the behavior regulation in most of the Gram-negative bacteria have widely been reported. This study aims to evaluate the effect of AHLs on Shewanella putrefaciens Z4 at the proteome level. It provides the theoretic basis for elucidating the spoilage mechanism of Shewanella spp., the common spoilage micro-organism in refrigerated marine aquatic food products.
希瓦氏菌属是低温储存的水产食品中常见的腐败菌，据报道其腐败机制由群体感应（QS）介导。然而，很少有关于响应N-酰基高丝氨酸内酯（AHLs）的特异性表达蛋白的报道。本研究旨在从蛋白质组水平评估不同AHL信号分子对从冷藏大菱鲆（Scophthalmus maximus）中分离出的腐败希瓦氏菌Z4的影响。结果表明，外源AHLs被腐败希瓦氏菌Z4用作QS信号分子，且AHLs不会被腐败希瓦氏菌Z4分泌的胞内或胞外酶降解。选择了添加AHLs后23个差异表达的斑点，并通过液相色谱-质谱联用（LC-MS）进行鉴定。结果表明，参与生长和代谢的蛋白质（如柠檬酸合酶、琥珀酸半醛脱氢酶）、环境适应和调节蛋白（如多糖脱乙酰酶、转醛醇酶）在三种AHLs（C4-HSL、C6-HSL和O-C6-HSL）作用下表达下调，而添加外源AHLs后应激反应蛋白和DNA连接酶的丰度升高。此外，外源C6-HSL和O-C6-HSL的作用显著。这些结果证明基于AHL的QS信号分子影响了腐败希瓦氏菌的一些重要代谢特性。
基于N-酰基高丝氨酸内酯（AHLs）的群体感应信号分子参与大多数革兰氏阴性菌的行为调控，已有广泛报道。本研究旨在从蛋白质组水平评估AHLs对腐败希瓦氏菌Z4的影响。它为阐明冷藏海洋水产食品中常见腐败微生物希瓦氏菌属的腐败机制提供了理论依据。
