Yu Honglei, Li Jianpeng, Han Yunyan, Shi Guocui, Liu Zunying, Zeng Mingyong
College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao, 266003 China.
J Food Sci Technol. 2019 Jan;56(1):114-121. doi: 10.1007/s13197-018-3464-8. Epub 2018 Nov 8.
is the predominant bacteria in spoiled shrimp (), however, the spoilage ability and the mechanism of is still unknown. can't produce the signal molecule of acyl-homoserine-lactones (AHLs), so the aim of this study was to investigate how wild type SA03 (WT SA03) eavesdrop exogenous AHLs to enhance its spoilage ability through LuxR receptor. The results indicated that spp. (Aer), spp. (Aci) and spp. (Ser) isolated from refrigerated shrimp can produce different AHLs. WT SA03 can eavesdrop the AHLs of Aer (C4-HSL), Aci (O-C6-HSL) and Ser (C6-HSL, O-C6-HSL) to enhance its growth, especially Ser. Exogenous C4-HSL and C6-HSL enhanced biofilm formation of WT SA03, and C6-HSL and O-C6-HSL enhanced thioredoxin reductase mRNA expression. However, the mutant of WT SA03 ( SA03) lost or weakened the role of using environmental AHLs. In vivo experiments, the lag time of WT SA03 was shortened by 6.4 h, 6.2 h and 14.4 h by co-inoculated with Aer, Aci and Ser, respectively. The total volatile basic nitrogen (TVB-N) were significantly enhanced in the samples co-inoculated with WT SA03 and Aer (or Aci, Ser) than those of SA03 and Aer (or Aci, Ser) (< 0.05). The results showed that SA03 can utilize AHLs produced by other bacteria to enhance its growth and spoilage ability through LuxR receptor system. Quorum sensing based on AHLs of bacteria might as the potential targets for food spoilage control.
是变质虾中的优势菌（），然而，其腐败能力和机制仍不清楚。不能产生酰基高丝氨酸内酯（AHLs）信号分子，因此本研究的目的是探讨野生型SA03（WT SA03）如何通过LuxR受体窃听外源AHLs以增强其腐败能力。结果表明，从冷藏虾中分离出的气单胞菌属（Aer）、不动杆菌属（Aci）和血清型（Ser）能产生不同的AHLs。WT SA03能窃听Aer（C4-HSL）、Aci（O-C6-HSL）和Ser（C6-HSL、O-C6-HSL）的AHLs以促进其生长，尤其是Ser。外源C4-HSL和C6-HSL增强了WT SA03的生物膜形成，C6-HSL和O-C6-HSL增强了硫氧还蛋白还原酶mRNA表达。然而，WT SA03的突变体（SA03）失去或削弱了利用环境AHLs的作用。在体内实验中，与Aer、Aci和Ser共接种时，WT SA03的延迟期分别缩短了6.4小时、6.2小时和14.4小时。与SA03和Aer（或Aci、Ser）共接种的样品相比，与WT SA03和Aer（或Aci、Ser）共接种的样品中的总挥发性盐基氮（TVB-N）显著增加（<0.05）。结果表明SA03可以通过LuxR受体系统利用其他细菌产生的AHLs来增强其生长和腐败能力。基于细菌AHLs的群体感应可能作为食品腐败控制的潜在靶点。
