Du Jing, Cao Mei-Da, Feng Shu-Li, Su Fang, Sang Xiao-Jing, Zhang Lan-Cui, You Wan-Sheng, Yang Mei, Zhu Zai-Ming
School of Chemistry and Chemical Engineering, Liaoning Normal University, Huanghe Road 850, Dalian, 116029, P.R. China.
Chemistry. 2017 Oct 17;23(58):14614-14622. doi: 10.1002/chem.201703158. Epub 2017 Sep 20.
Enzyme immobilization is of increasing importance for biocatalysis, for which good supports are critical. Herein, two new Preyssler-type polyoxometalate (POM)-based coordination polymers, namely, {[Cu(H biim) ][{Cu(H biim) (μ-H O)} Cu(H biim)(H O) ]H[({Cu(H biim)(H O) } ) ((μ-C HN Cl ){Cu(H biim)} ){Z(H O)P W O }]⋅x H O} (1: Z=Na, x=9; 2: Z=Ag, x=10; H biim=2,2'-biimidazole) were designed and synthesized. Compounds 1 and 2 exhibit the same skeletons, which contain multiple Cu complex fragments and penta-supported {ZP W } (Z=Na, Ag) clusters. They were first employed to immobilize horseradish peroxidase (HRP). Results show that compounds 1 and 2 are good supports for HRP immobilization, and exhibit higher enzyme loading, lower loading times, and excellent reusability. The immobilized HRP (HRP/1 or HRP/2) was further applied to detect H O , and good sensitivity, wide linear range, low detection limit, and fast response were achieved. This work shows that POM-based hybrid materials are a new kind of promising support for enzyme immobilization.
酶固定化对于生物催化越来越重要，为此良好的载体至关重要。在此，设计并合成了两种新型基于Preyssler型多金属氧酸盐（POM）的配位聚合物，即{[Cu(H biim) ][{Cu(H biim) (μ-H₂O)}₂Cu(H biim)(H₂O)₂ ]H[({Cu(H biim)(H₂O)₂ } )₂ ((μ-C₆H₄N₂Cl ){Cu(H biim)}₂ ){Z(H₂O)P₅W₁₀O₄₀ }]⋅x H₂O} （1：Z = Na，x = 9；2：Z = Ag，x = 10；H biim = 2,2'-联咪唑）。化合物1和2具有相同的骨架，其中包含多个铜配合物片段和五支撑的{ZP₅W₁₀ }（Z = Na，Ag）簇。它们首次被用于固定辣根过氧化物酶（HRP）。结果表明，化合物1和2是固定HRP的良好载体，并表现出更高的酶负载量、更短的负载时间和出色的可重复使用性。固定化的HRP（HRP/1或HRP/2）进一步用于检测H₂O₂ ，并实现了良好的灵敏度、宽线性范围、低检测限和快速响应。这项工作表明，基于POM的杂化材料是一种新型的有前途的酶固定化载体。
