Li Zongtai, Yang Tao, Li Xiaolei, Yin Panchao, Yang Bo, Li Dongying, Wang Yan, Teng Wei, Yu Qianqian, Li Weichang
Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, 510055, P. R. China.
South China Advanced Institute for Soft Matter Science and Technology, School of Emergent Soft Matter, Guangdong Provincial Key Laboratory of Functional and Intelligent Hybrid Materials and Devices, South China University of Technology, Guangzhou, 510640, P. R. China.
Adv Sci (Weinh). 2025 Jun;12(23):e2500088. doi: 10.1002/advs.202500088. Epub 2025 Apr 28.
Infectious tissue injuries, exacerbated by bacterial infections and antibiotic resistance, pose significant challenges for treatment and may lead to life-threatening systemic infections. In this study, a bio-responsive hydrogel system is developed, leveraging silver ions (Ag⁺) encapsulated in Preyssler-type polyoxometalates (POMs). The Ag⁺ ions are selectively released in response to endogenous sodium ions (Na⁺) within the biological environment, enabling broad-spectrum antibacterial activity. The POM serves as a protective matrix for Ag⁺, preserving its bioactivity while mitigating cytotoxicity and the reduction in antimicrobial efficacy associated with prolonged exposure. Additionally, a dual-channel technique is employed to fabricate fiber membranes with controllable and continuously stacked chemical compositions, ensuring efficient and uniform POM incorporation via hydrogen bonding within the fiber matrix. Subsequently, in situ hierarchical cross-linking process generated a spatially heterogeneous hydrogel with an interpenetrating network structure at multiple scales. This differentiated microstructure facilitates the controlled loading and release of diverse therapeutic components. Meanwhile, bioactive exosomes are integrated into the hydrogel, further enhancing its regenerative potential for treating infectious tissue injuries. In vitro and in vivo experiments demonstrated that the advanced hydrogel system provide a viable and efficient platform for addressing the challenges associated with infectious tissue injuries, offering a promising strategy for clinical applications.
由细菌感染和抗生素耐药性加剧的感染性组织损伤给治疗带来了重大挑战，并可能导致危及生命的全身感染。在本研究中，开发了一种生物响应性水凝胶系统，利用包裹在普雷施勒型多金属氧酸盐（POMs）中的银离子（Ag⁺）。Ag⁺离子在生物环境中对内源性钠离子（Na⁺）作出响应而选择性释放，从而实现广谱抗菌活性。POM作为Ag⁺的保护基质，在减轻细胞毒性以及与长时间暴露相关的抗菌效力降低的同时，保持其生物活性。此外，采用双通道技术制造具有可控且连续堆叠化学成分的纤维膜，通过纤维基质内的氢键确保POM的有效且均匀掺入。随后，原位分级交联过程产生了具有多尺度互穿网络结构的空间异质水凝胶。这种独特的微观结构有助于多种治疗成分的可控负载和释放。同时，将生物活性外泌体整合到水凝胶中，进一步增强其治疗感染性组织损伤的再生潜力。体外和体内实验表明，这种先进的水凝胶系统为应对与感染性组织损伤相关的挑战提供了一个可行且高效的平台，为临床应用提供了一种有前景的策略。
