Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, 223-8522, Japan.
Chem Asian J. 2017 Oct 18;12(20):2656-2659. doi: 10.1002/asia.201701004. Epub 2017 Sep 21.
A purpose-designed and synthesized H O -reactive and photoactivatable theranostic agent 1 consisting of 1) an arylboronic acid moiety, 2) pro-fluorophore moiety, and 3) photoactivatable moiety (photosensitizer), selectively and effectively reacted with H O while simultaneously releasing resorufin for fluorescence detection under neutral aqueous conditions. In addition, 1 was cell-permeable, and exhibited effective photocytotoxicity against fluorescently visualized cells only upon photoirradiation. The results also showed that 1 produced a selective fluorescence response to H O , even in living cultured cells.
一种专门设计和合成的 H2O2-反应性和光活化治疗剂 1，由 1）芳基硼酸部分，2）前荧光团部分和 3）光活化部分（光敏剂）组成，在中性水条件下选择性和有效地与 H2O2反应，同时释放用于荧光检测的 Resorufin。此外，1 具有细胞通透性，并仅在光照下表现出对荧光可视化细胞的有效光细胞毒性。结果还表明，1 对 H2O2产生了选择性荧光响应，即使在活的培养细胞中也是如此。
