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间质干细胞在多细胞生物工程三维体外肿瘤模型中的相关性。

Mesenchymal Stem Cells Relevance in Multicellular Bioengineered 3D In Vitro Tumor Models.

机构信息

Department of Chemistry, CICECO, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal.

Cancer Biology and Epigenetics Group, IPO Porto Research Center (CI-IPOP), Portuguese Oncology Institute of Porto (IPO Porto), Porto, Portugal.

出版信息

Biotechnol J. 2017 Dec;12(12). doi: 10.1002/biot.201700079. Epub 2017 Sep 4.

DOI:10.1002/biot.201700079
PMID:28834355
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7617208/
Abstract

In vitro 3D tumor microenvironment mimicking models are gathering momentum as alternatives to traditional 2D flat monolayer cultures due to their potential for recapitulating major cancer hallmarks. To fulfill such potential, it is crucial that 3D tumor testing platforms completely emulate in vitro the complex in vivo tumor niche and its cellular constituents. Mesenchymal stem cells (MSCs) are recognized to play a pivotal multi-modulatory role in cancer, generating interest as biological targets and as key tumor suppressing, or tumor promoting effectors. This review discusses the biological influence of different types of MSCs in the tumor microenvironment and showcases recent studies that engineer 3D MSCs-cancer cells co-cultures as advanced in vitro therapy testing platforms. A special focus is given to MSCs-cancer 3D co-culture set-up parameters, challenges, and future opportunities. Understanding cancer-MSCs crosstalk and their underlying effects is envisioned to support the development of advanced 3D in vitro disease models for discovery of forefront cancer treatments.

摘要

体外 3D 肿瘤微环境模拟模型由于其在重现主要癌症特征方面的潜力，正在成为传统 2D 单层培养物的替代方法。为了发挥这种潜力，3D 肿瘤测试平台必须完全模拟体外复杂的体内肿瘤生态位及其细胞成分。间充质干细胞 (MSCs) 被认为在癌症中发挥关键的多调节作用，作为生物靶点和关键的肿瘤抑制或肿瘤促进效应物引起了人们的兴趣。本综述讨论了不同类型的 MSCs 在肿瘤微环境中的生物学影响，并展示了最近将 3D MSC-癌细胞共培养作为先进的体外治疗测试平台进行工程设计的研究。特别关注 MSC-癌症 3D 共培养设置参数、挑战和未来机遇。预计对癌症-MSCs 串扰及其潜在影响的理解将支持先进的 3D 体外疾病模型的开发，以发现前沿的癌症治疗方法。

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本文引用的文献

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