Khorasani Mohammad M Y, Andam-Shahsavari Pouria, Zainodini Nahid, Khoramdelazad Hossein, Nosratabadi Reza
Department of Endodontics, School of Dentistry, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Immunology of Infectious Diseases Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
J Investig Clin Dent. 2018 Feb;9(1). doi: 10.1111/jicd.12290. Epub 2017 Aug 20.
S100 calcium-binding protein A1 (S100A12) is a pro-inflammatory molecule which is secreted during inflammation and induces chemotaxis and the production of pro-inflammatory cytokines via interaction with receptor for advanced glycation endproducts (RAGE) and subsequent, activation of nuclear factor-κB (NF-κB). The present study was designed to determine the expression levels of S100A12, RAGE, and NF-κB in the inflamed pulp of carried teeth.
In the present study, mRNA from 50 inflamed pulp and 50 healthy pulp were used for expression studies using real-time polymerase chain reaction. The expression levels of S100A12, RAGE, and NF-κB were compared between inflamed and healthy tissues.
The results revealed that the expression of S100A12, but not of RAGE or NF-κB, was significantly decreased in inflamed pulp when compared to healthy pulp. mRNA levels of RAGE were also increased in the inflamed pulp taken from men when compared with women.
The results suggest that S100A12 does not participate in the induction of inflammation in dental pulp. However, RAGE can participate in the inflammation in the pulp of males.
S100钙结合蛋白A1（S100A12）是一种促炎分子，在炎症过程中分泌，并通过与晚期糖基化终产物受体（RAGE）相互作用，随后激活核因子-κB（NF-κB）来诱导趋化作用和促炎细胞因子的产生。本研究旨在确定S100A12、RAGE和NF-κB在龋病牙髓炎症中的表达水平。
在本研究中，使用实时聚合酶链反应对50颗炎症牙髓和50颗健康牙髓的mRNA进行表达研究。比较炎症组织和健康组织中S100A12、RAGE和NF-κB的表达水平。
结果显示，与健康牙髓相比，炎症牙髓中S100A12的表达显著降低，而RAGE和NF-κB的表达无显著变化。与女性相比，男性炎症牙髓中RAGE的mRNA水平也有所升高。
结果表明，S100A12不参与牙髓炎症的诱导。然而，RAGE可能参与男性牙髓的炎症反应。
