Yaghooti Khorasani Mohammad Mahdi, Yousefi Amineh, Zainodini Nahid
Department of Endodontics, Faculty of Dental Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Immunology of Infectious Diseases Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
J Conserv Dent. 2019 Jan-Feb;22(1):12-16. doi: 10.4103/JCD.JCD_195_18.
Inflammation is a risk factor for dental complications. Inflammasomes are a set of intracellular sensors which participate in the induction of inflammation. As the main factors involved in the induction of pulp inflammation in the carious teeth are yet to be clarified, this study was aimed to evaluate NLRP1 and NLRC4 expression levels in the inflamed and healthy pulps.
Fifty inflamed and fifty healthy pulps were evaluated regarding the multiRNA levels of NLRP1 and NLRC4 using real-time polymerase chain reaction technique.
Results demonstrated that expression of NLRP1 ( = 0.985) and NLRC4 ( = 0.581) did not significantly differ in inflamed in comparison to healthy pulps. NLRP1 ( = 0.989) and NLRC4 ( = 0.170) did not change in males when compared with females in inflamed pulps. Furthermore, NLRP1 ( = 0.133) and NLRC4 ( = 0.642) were not altered in males in comparison to females in healthy pulps.
Although NLRP1 and NLRC4 are the main inflammasomes, it appears that they are not the responsible molecules involved in the human pulp inflammation in the carious teeth.
炎症是牙齿并发症的一个危险因素。炎性小体是一组参与炎症诱导的细胞内传感器。由于龋病牙髓炎症诱导的主要因素尚未明确，本研究旨在评估炎性牙髓和健康牙髓中NLRP1和NLRC4的表达水平。
采用实时聚合酶链反应技术，对50颗炎性牙髓和50颗健康牙髓的NLRP1和NLRC4多RNA水平进行评估。
结果表明，与健康牙髓相比，炎性牙髓中NLRP1（=0.985）和NLRC4（=0.581）的表达无显著差异。在炎性牙髓中，男性与女性相比，NLRP1（=0.989）和NLRC4（=0.170）没有变化。此外，在健康牙髓中，男性与女性相比，NLRP1（=0.133）和NLRC4（=0.642）没有改变。
虽然NLRP1和NLRC4是主要的炎性小体，但它们似乎不是龋病牙髓炎症的相关分子。
