Graham D Y, Klein P D, Evans D J, Evans D G, Alpert L C, Opekun A R, Boutton T W
Lancet. 1987 May 23;1(8543):1174-7. doi: 10.1016/s0140-6736(87)92145-3.
The high endogenous urease activity of Campylobacter pylori was exploited in a non-invasive test for the presence of this organism in the stomach. When 13C-urea was administered orally after a test meal, urea-derived 13CO2 appeared in the respiratory CO2 of infected individuals at a constant rate for greater than 100 min. The test was validated in 26 individuals who underwent both the 13C-urea breath test and endoscopic biopsy of the antral mucosa for culture and histological examination. Each positive breath test proved to be correlated with a positive culture or Warthin-Starry silver stain of a mucosal biopsy specimen, or both.
幽门螺杆菌的高内源性脲酶活性被用于一种非侵入性检测，以检测胃中是否存在这种微生物。在试餐后口服13C-尿素时，感染个体呼出的二氧化碳中尿素衍生的13CO2会以恒定速率持续出现超过100分钟。该检测在26名个体中得到验证，这些个体同时接受了13C-尿素呼气试验以及胃窦黏膜内镜活检以进行培养和组织学检查。每次呼气试验阳性都被证明与黏膜活检标本的培养阳性或Warthin-Starry银染色阳性，或两者均阳性相关。
