• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

比较转录组分析揭示了抗氧化基因在两种白菜品种响应镉胁迫中的作用。

Comparative transcriptomic analysis reveals the roles of ROS scavenging genes in response to cadmium in two pak choi cultivars.

机构信息

College of Life Sciences, Huaibei Normal University, Huaibei, Anhui, 235000, P.R. China.

出版信息

Sci Rep. 2017 Aug 23;7(1):9217. doi: 10.1038/s41598-017-09838-2.

DOI:10.1038/s41598-017-09838-2
PMID:28835647
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5569009/
Abstract

To identify key regulatory genes involved in ROS scavenging in response to cadmium (Cd) exposure in pak choi, eight cDNA libraries from Cd-treated and Cd-free roots of two cultivars, Baiyewuyueman (high Cd accumulator) and Kuishan'aijiaoheiye (low Cd accumulator), were firstly performed by RNA-sequencing. Totally 0.443 billion clean reads and 244,190 unigenes were obtained from eight transcriptome. About 797 and 1167 unigenes encoding ROS related proteins and transcription factors were identified. Of them, 11 and 16 ROS scavenging system related DEGs, and 29 and 15 transcription factors related DEGs were found in Baiyewuyueman and Kuishan'aijiaoheiye, respectively. Ten ROS-scavenging genes (Cu/Zn-SOD, GST1, PODs, TrxR2, PrxR, FER3 and NDPK) showed higher expression levels in Cd-exposed seedings of Baiyewuyueman than those of Kuishan'aijiaoheiye. Four genes (GPX, APX, GRX and GST3) specifically expressed in Cd-free roots of Kuishan'aijiaoheiye. For transcription factors, ERF12/13/22 and WRKY31 was up-regulated by Cd in Baiyewuyueman, while in Kuishan'aijiaoheiye, Cd induced down-regulations of bZIP, NAC and ZFP families. The results indicate that the two cultivars differed in the mechanism of ROS scavenging in response to Cd stress. Fe SOD1, POD A2/44/54/62 and GST1 may be responsible for the difference of Cd tolerance between Baiyewuyueman and Kuishan'aijiaoheiye.

摘要

为了鉴定白菜响应镉胁迫时参与活性氧（ROS）清除的关键调控基因，本研究首先通过 RNA 测序构建了两个品种（镉高积累品种白优 5 号和镉低积累品种快山矮脚黄）镉处理和无镉根的 8 个 cDNA 文库。从 8 个转录组中共获得 0.443 亿条清洁reads 和 244190 个 unigenes。鉴定出约 797 个和 1167 个编码 ROS 相关蛋白和转录因子的 unigenes。其中，在白优 5 号和快山矮脚黄中分别发现了 11 个和 16 个 ROS 清除系统相关的差异表达基因（DEGs），以及 29 个和 15 个转录因子相关的 DEGs。10 个 ROS 清除基因（Cu/Zn-SOD、GST1、PODs、TrxR2、PrxR、FER3 和 NDPK）在镉暴露的白优 5 号种子中的表达水平高于快山矮脚黄。4 个基因（GPX、APX、GRX 和 GST3）在无镉处理的快山矮脚黄根中特异性表达。对于转录因子，ERF12/13/22 和 WRKY31 在白优 5 号中被 Cd 诱导上调，而在快山矮脚黄中，Cd 诱导 bZIP、NAC 和 ZFP 家族下调。研究结果表明，两个品种在响应 Cd 胁迫时 ROS 清除的机制存在差异。Fe-SOD1、POD A2/44/54/62 和 GST1 可能是白优 5 号和快山矮脚黄镉耐性差异的原因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7a6/5569009/c188c563604f/41598_2017_9838_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7a6/5569009/a973d804deef/41598_2017_9838_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7a6/5569009/8426eec6a69b/41598_2017_9838_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7a6/5569009/ccbd8ee8c949/41598_2017_9838_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7a6/5569009/c6b845084a29/41598_2017_9838_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7a6/5569009/c188c563604f/41598_2017_9838_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7a6/5569009/a973d804deef/41598_2017_9838_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7a6/5569009/8426eec6a69b/41598_2017_9838_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7a6/5569009/ccbd8ee8c949/41598_2017_9838_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7a6/5569009/c6b845084a29/41598_2017_9838_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7a6/5569009/c188c563604f/41598_2017_9838_Fig5_HTML.jpg

相似文献

1
Comparative transcriptomic analysis reveals the roles of ROS scavenging genes in response to cadmium in two pak choi cultivars.比较转录组分析揭示了抗氧化基因在两种白菜品种响应镉胁迫中的作用。
Sci Rep. 2017 Aug 23;7(1):9217. doi: 10.1038/s41598-017-09838-2.
2
Comparative transcriptome analysis reveals key cadmium transport-related genes in roots of two pak choi (Brassica rapa L. ssp. chinensis) cultivars.比较转录组分析揭示了两个小白菜（Brassica rapa L. ssp. chinensis）品种根系中与镉转运相关的关键基因。
BMC Genomics. 2017 Aug 8;18(1):587. doi: 10.1186/s12864-017-3973-2.
3
Comparative Transcriptome Analysis of the Molecular Mechanism of the Hairy Roots of L. in Response to Cadmium Stress.比较转录组分析镉胁迫下 L. 毛状根分子机制。
Int J Mol Sci. 2019 Dec 26;21(1):180. doi: 10.3390/ijms21010180.
4
Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars.比较转录组分析揭示了两个不同花生品种根系中镉差异运输和保留的关键因素。
BMC Genomics. 2018 Dec 17;19(1):938. doi: 10.1186/s12864-018-5304-7.
5
Co-expression network analysis of the transcriptomes of rice roots exposed to various cadmium stresses reveals universal cadmium-responsive genes.对暴露于各种镉胁迫下的水稻根系转录组进行共表达网络分析，揭示了普遍的镉响应基因。
BMC Plant Biol. 2017 Nov 7;17(1):194. doi: 10.1186/s12870-017-1143-y.
6
The zinc-regulated protein (ZIP) family genes and glutathione s-transferase (GST) family genes play roles in Cd resistance and accumulation of pak choi (Brassica campestris ssp. chinensis).锌调节蛋白（ZIP）家族基因和谷胱甘肽 S-转移酶（GST）家族基因在白菜（ Brassica campestris ssp. chinensis ）的 Cd 抗性和积累中发挥作用。
Ecotoxicol Environ Saf. 2019 Nov 15;183:109571. doi: 10.1016/j.ecoenv.2019.109571. Epub 2019 Aug 22.
7
Comparative transcriptome analysis reveals gene network regulating cadmium uptake and translocation in peanut roots under iron deficiency.比较转录组分析揭示了缺铁条件下花生根系镉吸收和转运的基因调控网络。
BMC Plant Biol. 2019 Jan 21;19(1):35. doi: 10.1186/s12870-019-1654-9.
8
Transcriptome analysis revealed pivotal transporters involved in the reduction of cadmium accumulation in pak choi (Brassica chinensis L.) by exogenous hydrogen-rich water.转录组分析揭示了外源性富氢水降低小白菜（ Brassica chinensis L. ）中镉积累的关键转运蛋白。
Chemosphere. 2019 Feb;216:684-697. doi: 10.1016/j.chemosphere.2018.10.152. Epub 2018 Oct 25.
9
Diverse strategies conferring extreme cadmium (Cd) tolerance in the dark septate endophyte (DSE), Exophiala pisciphila: evidence from RNA-seq data.多种策略赋予黑暗有隔内生菌（DSE）嗜鱼外瓶霉对极端镉（Cd）的耐受性：来自RNA测序数据的证据
Microbiol Res. 2015 Jan;170:27-35. doi: 10.1016/j.micres.2014.09.005. Epub 2014 Sep 28.
10
Osmoregulation and antioxidant production in maize under combined cadmium and arsenic stress.镉砷复合胁迫下玉米的渗透调节和抗氧化产物生成。
Environ Sci Pollut Res Int. 2016 Jun;23(12):11864-75. doi: 10.1007/s11356-016-6382-1. Epub 2016 Mar 9.

引用本文的文献

1
Molecular Mechanisms of Cadmium Stress Resistance in Vegetable Crops.蔬菜作物镉胁迫抗性的分子机制
Int J Mol Sci. 2025 Jun 17;26(12):5812. doi: 10.3390/ijms26125812.
2
Overexpression of Improved Cadmium Accumulation and Tolerance in Tobacco ( L.).提高烟草（L.）镉积累和耐受性的过表达。
Genes (Basel). 2024 Mar 15;15(3):366. doi: 10.3390/genes15030366.
3
Identification and Validation of Reference Genes for RT-qPCR Analysis in Reed Canary Grass during Abiotic Stress.雀麦草非生物胁迫下 RT-qPCR 分析中参考基因的鉴定和验证。

本文引用的文献

1
Overexpressing the Cu/Zn Superoxide Dismutase Increased Resistance to Oxidative Stress in Transgenic .过表达铜锌超氧化物歧化酶可增强转基因植物对氧化应激的抗性。
Front Plant Sci. 2017 Jun 13;8:1010. doi: 10.3389/fpls.2017.01010. eCollection 2017.
2
Functional analyses of NDPK2 in Populus trichocarpa and overexpression of PtNDPK2 enhances growth and tolerance to abiotic stresses in transgenic poplar.毛果杨中NDPK2的功能分析及PtNDPK2的过表达增强了转基因杨树的生长和对非生物胁迫的耐受性。
Plant Physiol Biochem. 2017 Aug;117:61-74. doi: 10.1016/j.plaphy.2017.05.019. Epub 2017 May 31.
3
GmWRKY31 and GmHDL56 Enhances Resistance to by Regulating Defense-Related Gene Expression in Soybean.
Genes (Basel). 2023 Sep 12;14(9):1790. doi: 10.3390/genes14091790.
4
Exploring the Biologically Active Metabolites Produced by for Plant Growth Promotion, Heat Stress Tolerance, and Resistance to Bacterial Soft Rot in .探索[具体对象]产生的具有生物活性的代谢产物，用于促进植物生长、提高耐热性以及增强对[具体植物]细菌性软腐病的抗性。
Metabolites. 2023 May 22;13(5):676. doi: 10.3390/metabo13050676.
5
Identification of genetic loci associated with five agronomic traits in alfalfa using multi-environment trials.利用多环境试验鉴定苜蓿五个农艺性状相关的遗传位点
Theor Appl Genet. 2023 Apr 29;136(5):121. doi: 10.1007/s00122-023-04364-4.
6
Cadmium toxicity in medicinal plants: An overview of the tolerance strategies, biotechnological and omics approaches to alleviate metal stress.药用植物中的镉毒性：耐受策略、缓解金属胁迫的生物技术和组学方法概述
Front Plant Sci. 2023 Jan 17;13:1047410. doi: 10.3389/fpls.2022.1047410. eCollection 2022.
7
A Comprehensive Analysis of the DUF4228 Gene Family in Reveals the Role of in Salt Tolerance.在 中全面分析 DUF4228 基因家族揭示了 在耐盐性中的作用。
Int J Mol Sci. 2022 Nov 4;23(21):13542. doi: 10.3390/ijms232113542.
8
Overexpression of Transcription Factor from Improves Plant Salt Tolerance.转录因子的过表达提高了植物的耐盐性。
Int J Mol Sci. 2022 Jul 17;23(14):7879. doi: 10.3390/ijms23147879.
9
Transcriptomic Analysis Revealed Reactive Oxygen Species Scavenging Mechanisms Associated With Ferrous Iron Toxicity in Aromatic Keteki Joha Rice.转录组学分析揭示了香稻凯泰基乔哈中铁毒性相关的活性氧清除机制。
Front Plant Sci. 2022 Feb 24;13:798580. doi: 10.3389/fpls.2022.798580. eCollection 2022.
10
Transcriptomic Analysis Reveals the Roles of Detoxification Systems in Response to Mercury in .转录组分析揭示了解毒系统在应对汞中的作用。
Biomolecules. 2019 Oct 24;9(11):647. doi: 10.3390/biom9110647.
GmWRKY31和GmHDL56通过调控大豆中与防御相关的基因表达增强对[具体病害或其他因素]的抗性。 （注：原文中“by Regulating Defense-Related Gene Expression in Soybean”部分前面缺少具体所针对的对象，这里只能按大概意思补充完整）
Front Plant Sci. 2017 May 12;8:781. doi: 10.3389/fpls.2017.00781. eCollection 2017.
4
Overexpression of Induces Orchestrated Reactive Oxygen Scavenging and Enhances Cold and Heat Tolerances in Tobacco.[基因名称]的过表达诱导烟草中有序的活性氧清除并增强其耐寒性和耐热性。 （这里原文Induces前面缺少具体基因名称，根据实际情况补充即可）
Biomed Res Int. 2017;2017:4049534. doi: 10.1155/2017/4049534. Epub 2017 Mar 13.
5
Overexpression of wheat ferritin gene TaFER-5B enhances tolerance to heat stress and other abiotic stresses associated with the ROS scavenging.小麦铁蛋白基因TaFER-5B的过表达增强了对热胁迫及与活性氧清除相关的其他非生物胁迫的耐受性。
BMC Plant Biol. 2017 Jan 14;17(1):14. doi: 10.1186/s12870-016-0958-2.
6
The role of ZmWRKY4 in regulating maize antioxidant defense under cadmium stress.ZmWRKY4在镉胁迫下调控玉米抗氧化防御中的作用。
Biochem Biophys Res Commun. 2017 Jan 22;482(4):1504-1510. doi: 10.1016/j.bbrc.2016.12.064. Epub 2016 Dec 9.
7
Editorial: ROS Regulation during Plant Abiotic Stress Responses.社论：植物非生物胁迫响应过程中的活性氧调节
Front Plant Sci. 2016 Oct 19;7:1536. doi: 10.3389/fpls.2016.01536. eCollection 2016.
8
RNA-seq analysis revealed ROS-mediated related genes involved in cadmium detoxification in the razor clam Sinonovacula constricta.RNA测序分析揭示了缢蛏中参与镉解毒的活性氧介导相关基因。
Fish Shellfish Immunol. 2016 Oct;57:350-361. doi: 10.1016/j.fsi.2016.08.051. Epub 2016 Aug 26.
9
Overexpression of a novel NAC-type tomato transcription factor, SlNAM1, enhances the chilling stress tolerance of transgenic tobacco.一种新型NAC类番茄转录因子SlNAM1的过表达增强了转基因烟草的耐冷胁迫能力。
J Plant Physiol. 2016 Oct 1;204:54-65. doi: 10.1016/j.jplph.2016.06.024. Epub 2016 Jul 30.
10
Ethylene Response Factor TERF1, Regulated by ETHYLENE-INSENSITIVE3-like Factors, Functions in Reactive Oxygen Species (ROS) Scavenging in Tobacco (Nicotiana tabacum L.).乙烯应答因子 TERF1 通过受 ETHYLENE-INSENSITIVE3 样因子调控，在烟草（Nicotiana tabacum L.）活性氧（ROS）清除中发挥作用。
Sci Rep. 2016 Jul 20;6:29948. doi: 10.1038/srep29948.