Li Zhen, Han Xiaojiao, Song Xixi, Zhang Yunxing, Jiang Jing, Han Qiang, Liu Mingying, Qiao Guirong, Zhuo Renying
State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of ForestryBeijing, China.
Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical of Forestry, Chinese Academy of ForestryHangzhou, China.
Front Plant Sci. 2017 Jun 13;8:1010. doi: 10.3389/fpls.2017.01010. eCollection 2017.
Superoxide dismutase (SOD) is a very important reactive oxygen species (ROS)-scavenging enzyme. In this study, the functions of a gene (), from , a cadmium (Cd)/zinc/lead co-hyperaccumulator of the Crassulaceae, was characterized. The expression of was induced by Cd stress. Compared with wild-type (WT) plants, overexpression of gene in transgenic plants enhanced the antioxidative defense capacity, including SOD and peroxidase activities. Additionally, it reduced the damage associated with the overproduction of hydrogen peroxide (HO) and superoxide radicals (O). The influence of Cd stress on ion flux across the root surface showed that overexpressing in transgenic plants has greater Cd uptake capacity existed in roots. A co-expression network based on microarray data showed possible oxidative regulation in after Cd-induced oxidative stress, suggesting that S may participate in this network and enhance ROS-scavenging capability under Cd stress. Taken together, these results suggest that overexpressing increased oxidative stress resistance in transgenic and provide useful information for understanding the role of in response to abiotic stress.
超氧化物歧化酶（SOD）是一种非常重要的活性氧（ROS）清除酶。在本研究中，对来自景天科镉（Cd）/锌/铅共超积累植物的一个基因（）的功能进行了表征。该基因的表达受镉胁迫诱导。与野生型（WT）植物相比，该基因在转基因植物中的过表达增强了抗氧化防御能力，包括超氧化物歧化酶和过氧化物酶活性。此外，它减少了与过氧化氢（HO）和超氧自由基（O）过量产生相关的损伤。镉胁迫对根表面离子通量的影响表明，在转基因植物中过表达该基因后根系具有更强的镉吸收能力。基于微阵列数据的共表达网络显示，镉诱导的氧化应激后该基因可能存在氧化调控，这表明该基因可能参与此网络并在镉胁迫下增强ROS清除能力。综上所述，这些结果表明该基因过表达提高了转基因植物的氧化应激抗性，并为理解该基因在应对非生物胁迫中的作用提供了有用信息。
