Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, People's Republic of China.
School of Pharmacy, Wenzhou Medical University, Chashan University Park, Wenzhou, 325035, People's Republic of China.
Bioprocess Biosyst Eng. 2017 Nov;40(11):1689-1699. doi: 10.1007/s00449-017-1824-9. Epub 2017 Aug 24.
Lipase-displaying yeast cells are a promising alternative to the conventional immobilised lipases for organic bioconversions. However, the hydrophilic characteristics of the yeast cell surface may impede efficient immobilisation. Herein, we tested three methods to enhance the hydrophobicity of the surface of Candida antarctica lipase B-displaying Pichia pastoris cells, co-displaying a fungal hydrophobin, coating with ionic liquids, and adding decane as a hydrophobic carbon source during fermentation. Modified cells showed higher surface hydrophobicity and superior esterification of C-C saturated fatty acids in hydrophobic solvents. When used for biodiesel synthesis, modified cells exhibited an improved initial reaction rate and equilibrium fatty acid methyl ester yield. We systematically discuss the influence of cell surface hydrophobicity on the catalytic properties, and the results provide guidance for improving the catalytic efficiency and operational characteristics of lipase-displaying yeast cells for organic bioconversions.
展示脂肪酶的酵母细胞是一种有前途的替代传统固定化脂肪酶进行有机生物转化的方法。然而，酵母细胞表面的亲水性可能会阻碍有效的固定化。本文中，我们测试了三种方法来增强展示南极假丝酵母脂肪酶的巴斯德毕赤酵母细胞的表面疏水性，分别是共展示真菌疏水蛋白、用离子液体包被和在发酵过程中添加癸烷作为疏水碳源。改性细胞表现出更高的表面疏水性和在疏水性溶剂中对 C-C 饱和脂肪酸的酯化作用更好。当用于生物柴油合成时，改性细胞表现出改善的初始反应速率和平衡脂肪酸甲酯产率。我们系统地讨论了细胞表面疏水性对催化性能的影响，结果为提高展示脂肪酶的酵母细胞在有机生物转化中的催化效率和操作特性提供了指导。
