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静态磁场影响下新鲜和玻璃化小鼠卵巢异体移植至睾丸组织的比较。

Comparison of Allotransplantation of Fresh and Vitrified Mouse Ovaries to The Testicular Tissue under Influence of The Static Magnetic Field.

作者信息

Kazemein Jasemi Vida Sadat, Samadi Firooz, Eimani Hussein, Hasani Saeed, Fathi Rouhollah, Shahverdi Abdolhossein

机构信息

Department of Animal and Poultry Physiology, Faculty of Animal Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Golestan, Iran.

Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.

出版信息

Cell J. 2017 Oct;19(3):492-505. doi: 10.22074/cellj.2017.4513. Epub 2017 Aug 19.

DOI:10.22074/cellj.2017.4513
PMID:28836412
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5570414/
Abstract

OBJECTIVES

The aim of this study was to investigate the effects of static magnetic field (SMF) during transplantation of the ovarian tissue into the testis.

MATERIALS AND METHODS

In this experimental study, ovaries of 6- to 8-week-old female Naval Medical Research Institute (NMRI) mice were randomly divided into four groups: i. Fresh ovaries were immediately transplanted into the testicular tissue (FOT group), ii. Fresh ovaries were exposed to the SMF for 10 minutes and then transplanted into the testicular tissue (FOT+ group), iii. Vitrified-warmed ovaries were transplanted into the testicular tissue (VOT group), and iv. Vitrified-warmed ovaries were transplanted into the testicular tissue and the transplantation site was then exposed to the SMF for 10 minutes (VOT+ group).

RESULTS

The lowest percentages of morphologically dead primordial follicles and the highest percentages of morphologically intact primordial follicles were seen in the FOT+ group (4.11% ± 2.88 and 41.26% ± 0.54, respectively). Although the lowest significant percentage of maturation, embryonic development and fertility was observed in the VOT group as compared to the other groups, the difference in the fertility rate was not significant between the VOT and VOT+ groups. Estrogen and progesterone concentrations were significantly higher in the FOT+ group than those of the control mice.

CONCLUSIONS

It is concluded that, exposure of the vitrified-warmed ovaries to SMF retains the structure of the graft similar to that of fresh ovaries.

摘要

目的

本研究旨在探讨卵巢组织移植至睾丸过程中静磁场（SMF）的作用。

材料与方法

在本实验研究中，将6至8周龄雌性海军医学研究所（NMRI）小鼠的卵巢随机分为四组：i. 新鲜卵巢立即移植至睾丸组织（FOT组）；ii. 新鲜卵巢暴露于静磁场10分钟后移植至睾丸组织（FOT+组）；iii. 玻璃化冷冻复温后的卵巢移植至睾丸组织（VOT组）；iv. 玻璃化冷冻复温后的卵巢移植至睾丸组织，然后移植部位暴露于静磁场10分钟（VOT+组）。

结果

FOT+组中形态学上死亡的原始卵泡百分比最低，形态学上完整的原始卵泡百分比最高（分别为4.11%±2.88和41.26%±0.54）。尽管与其他组相比，VOT组观察到的成熟、胚胎发育和生育能力的最低显著百分比，但VOT组和VOT+组之间的生育率差异不显著。FOT+组中的雌激素和孕酮浓度显著高于对照小鼠。

结论

得出结论，玻璃化冷冻复温后的卵巢暴露于静磁场可使移植物结构保持与新鲜卵巢相似。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/709e/5570414/69976300c2d3/Cell-J-19-492-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/709e/5570414/fbc8b0032175/Cell-J-19-492-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/709e/5570414/f0ab9e86c133/Cell-J-19-492-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/709e/5570414/9d970b165e1b/Cell-J-19-492-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/709e/5570414/4d1000d4acfc/Cell-J-19-492-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/709e/5570414/69976300c2d3/Cell-J-19-492-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/709e/5570414/fbc8b0032175/Cell-J-19-492-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/709e/5570414/f0ab9e86c133/Cell-J-19-492-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/709e/5570414/9d970b165e1b/Cell-J-19-492-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/709e/5570414/4d1000d4acfc/Cell-J-19-492-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/709e/5570414/69976300c2d3/Cell-J-19-492-g05.jpg

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Effect of therapeutic ultrasound on folliculogenesis, angiogenesis and apoptosis after heterotopic mouse ovarian transplantation.
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Ultrasound Med Biol. 2014 Jul;40(7):1535-44. doi: 10.1016/j.ultrasmedbio.2014.02.006. Epub 2014 Apr 27.
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In vitro maturation, fertilization and embryo culture of oocytes obtained from vitrified auto-transplanted mouse ovary.从玻璃化自体移植小鼠卵巢获得的卵母细胞的体外成熟、受精及胚胎培养
Int J Fertil Steril. 2013 Jan;6(4):278-85. Epub 2013 Mar 3.
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