Sato Masahiro, Sakurai Takayuki, Kiryu Kazunori, Takeda Masaki, Yasuoka Yukiko
Division of Basic Molecular Science and Molecular Medicine, School of Medicine, Tokai University and.
Department of Physiology, Kitazato University School of Medicine, Kanagawa, Japan.
Reprod Med Biol. 2006 Mar 1;5(1):71-77. doi: 10.1111/j.1447-0578.2006.00126.x. eCollection 2006 Mar.
To examine the effects of intratesticular transplantation on the growth and maturation of young murine ovaries. Two-week-old ovaries from transgenic mice with enhanced green fluorescent protein expression were transplanted under the testicular capsule of 4-week-old non-transgenic mice. Two months after transplantation all successfully grafted ovaries had survived, based on the presence of bright green fluorescence. The grafts showed various stages of folliculogenesis, including expanded follicles. The neighboring seminiferous tubules had a normal structure and mature sperm in their lumens, indicating active spermatogenesis, and all the recipient males were fertile. There was no evidence of extensive cell migration from the grafted ovaries into the testis. Similar findings were obtained for the grafted ovaries 6 months after surgery, although cell death (as evidenced by yellowish or pale fluorescence) was more frequent. Young murine ovaries can grow and mature autonomously for at least 6 months unaffected by the male hormonal environment. (Reprod Med Biol 2006; : 71-77).
为研究睾丸内移植对幼年小鼠卵巢生长和成熟的影响。将来自增强型绿色荧光蛋白表达转基因小鼠的两周龄卵巢移植到四周龄非转基因小鼠的睾丸被膜下。移植两个月后，基于明亮的绿色荧光，所有成功移植的卵巢均存活。移植的卵巢呈现出不同阶段的卵泡发生，包括扩张的卵泡。相邻的生精小管结构正常，管腔内有成熟精子，表明精子发生活跃，所有受体雄性均具有生育能力。没有证据表明移植的卵巢有大量细胞迁移到睾丸中。术后6个月对移植卵巢的观察也得到了类似结果，尽管细胞死亡（表现为淡黄色或淡绿色荧光）更为常见。幼年小鼠卵巢能够自主生长和成熟至少6个月，不受雄性激素环境的影响。（《生殖医学与生物学》2006年；：71 - 77）
