Sun Dan, Lu Yao, Zhang Su-Juan, Wang Kai-Ge, Li Yuan
National Center for International Research of Photoelectric Technology and Nano-functional Materials, State Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials, Key Laboratory of Optoelectronic Technology of Shaanxi Province, Institute of Photonics and Photo-Technology, Northwest University, Xi'an, 710069, PR China.
Gen Physiol Biophys. 2018 May;37(3):319-328. doi: 10.4149/gpb_2017043.
Ellagic acid (EA) is a naturally phenolic acid presented in different foods. It has a variety of biological activities including antioxidant, anti-inflammatory, anti-microbiological and anti-cancer properties. On account of its antioxidant activity, EA might protect cancer cells from free radical damage in photodynamic therapy (PDT) during which reactive oxygen species (ROS) production was stimulated leading to irreversible tumor cell injury. In this study, the influence of EA on K562 cells in 5-aminolevulinic acid (ALA)-based PDT is demonstrated. Cell apoptosis was assayed by flow cytometry. Oxidative damage induced by PDT was investigated by measurement of malondialdehyde (MDA). Comet assay was used to evaluate the potential genotoxic effect induced by PDT on the cells. The results showed that EA supplementation alone did not affect the lipid peroxidation, DNA damage and apoptosis in K562 cells. It increases the lipid peroxidation, DNA damage, apoptosis and decreases the survival rate in K562 cells induced by ALA-PDT. The singlet oxygen quencher sodium azide suppresses apoptosis, lipid peroxidation and DNA damage induced by EA in PDT. In conclusion, EA consumption during PDT did not decrease the effectiveness of cancer therapy on malignant cells. The effect of antioxidants on PDT maybe was determined by its sensitization ability to singlet oxygen.
鞣花酸（EA）是一种存在于不同食物中的天然酚酸。它具有多种生物活性，包括抗氧化、抗炎、抗微生物和抗癌特性。由于其抗氧化活性，EA可能会在光动力疗法（PDT）中保护癌细胞免受自由基损伤，在此过程中会刺激活性氧（ROS）的产生，从而导致不可逆的肿瘤细胞损伤。在本研究中，证明了EA对基于5-氨基乙酰丙酸（ALA）的PDT中K562细胞的影响。通过流式细胞术检测细胞凋亡。通过测量丙二醛（MDA）来研究PDT诱导的氧化损伤。彗星试验用于评估PDT对细胞诱导的潜在遗传毒性作用。结果表明，单独补充EA不会影响K562细胞中的脂质过氧化、DNA损伤和细胞凋亡。它会增加ALA-PDT诱导的K562细胞中的脂质过氧化、DNA损伤、细胞凋亡，并降低其存活率。单线态氧猝灭剂叠氮化钠可抑制PDT中EA诱导的细胞凋亡、脂质过氧化和DNA损伤。总之，在PDT期间食用EA不会降低对恶性细胞的癌症治疗效果。抗氧化剂对PDT的影响可能取决于其对单线态氧的敏化能力。
