Bayat Bahareh, Zade Masoumeh Hallaj, Mansouri Samaneh, Kalantar Enayat, Kabir Kourosh, Zahmatkesh Ehsan, Sepehr Mohammad Noori, Naseri Mohammmad Hassan, Darban-Sarokhalil Davood
1 Department of Microbiology, School of Medicine, Iran University of Medical Sciences , Tehran, Iran.
2 Department of Microbiology, School of Medicine, Alborz University of Medical Sciences , Karaj, Iran.
Acta Microbiol Immunol Hung. 2017 Sep 1;64(3):331-341. doi: 10.1556/030.64.2017.020. Epub 2017 Aug 24.
Methicillin-resistant Staphylococcus aureus (MRSA) has been one of the most important antibiotic-resistant pathogen in many parts of the world over the past decades. This cross-sectional study was conducted to investigate MRSA isolated between July 2013 and July 2014 in Karaj, Iran. All tested isolates were collected in teaching hospitals from personnel, patients, and surfaces and each MRSA was analyzed by SCCmec and spa typing. Antibiotic susceptibility testing was accomplished by disk diffusion method. Out of 49 MRSA isolates from the Karaj's teaching hospitals, 82%, 10%, and 6% of the isolates were SCCmec types III, II, and I, respectively. The main spa type in this study was spa t030 with frequency as high as 75.5% from intensive care unit (ICU) of the hospitals and high rate of resistance to rifampicin (53%) was found in MRSA isolates. In conclusion, high frequency of spa t030 with SCCmec type III and MRSA phenotype illustrated circulating of one of the antibiotic-resistant strains in ICU of Karaj's teaching hospitals and emphasizes the need for ongoing molecular surveillance, antibiotic susceptibility monitoring, and infection control.
在过去几十年里，耐甲氧西林金黄色葡萄球菌（MRSA）一直是世界许多地区最重要的耐药病原体之一。本横断面研究旨在调查2013年7月至2014年7月间在伊朗卡拉季分离出的MRSA。所有测试菌株均从教学医院的人员、患者和表面采集，每株MRSA均通过SCCmec和spa分型进行分析。采用纸片扩散法进行药敏试验。在卡拉季教学医院分离出的49株MRSA中，分别有82%、10%和6%的菌株为SCCmec III型、II型和I型。本研究中主要的spa型为spa t030，在医院重症监护病房（ICU）中的出现频率高达75.5%，并且在MRSA菌株中发现对利福平的耐药率很高（53%）。总之，spa t030与SCCmec III型和MRSA表型的高频率表明卡拉季教学医院ICU中存在一种耐药菌株，强调了持续进行分子监测、药敏监测和感染控制的必要性。
