生长抑素对分离的豚鼠胃腺内因子分泌的抑制作用。

Somatostatin inhibition of intrinsic factor secretion from isolated guinea pig gastric glands.

作者信息

Oddsdottir M, Ballantyne G H, Adrian T E, Zdon M J, Zucker K A, Modlin I M

出版信息

Scand J Gastroenterol. 1987 Mar;22(2):233-8. doi: 10.3109/00365528708991885.

Abstract

The aim of our study was to examine the direct effect of somatostatin on histamine- and pentagastrin-stimulated intrinsic factor (IF) release in collagenase-dispersed guinea pig gastric glands. The effect of somatostatin (10(-11) M to 10(-6) M) on half-maximal doses of histamine (10(-6) M), pentagastrin (10(-6) M), and both histamine and pentagastrin together was tested. All tested concentrations of histamine significantly stimulated IF release. Pentagastrin (10(-10) M to 10(-6) M) inconsistently stimulated IF release. The quantity of IF release stimulated by histamine and pentagastrin together was approximately the additive sum of that produced by either agent alone. Somatostatin (10(-6) M) inhibited histamine-stimulated (10(-6) M) IF release by 69.9 +/- 7.2% and the combination of histamine (10(-6) M) and pentagastrin (10(-6) M) by 64.2 +/- 9.1%. This is the first in vitro demonstration that somatostatin inhibits IF release.

摘要

我们研究的目的是检测生长抑素对胶原酶分散的豚鼠胃腺中组胺和五肽胃泌素刺激的内因子（IF）释放的直接作用。测试了生长抑素（10⁻¹¹ M至10⁻⁶ M）对组胺（10⁻⁶ M）、五肽胃泌素（10⁻⁶ M）半最大剂量以及组胺和五肽胃泌素共同作用的影响。所有测试浓度的组胺均显著刺激IF释放。五肽胃泌素（10⁻¹⁰ M至10⁻⁶ M）对IF释放的刺激作用不一致。组胺和五肽胃泌素共同刺激的IF释放量约为单独使用任一药物所产生量的加和。生长抑素（10⁻⁶ M）抑制组胺（10⁻⁶ M）刺激的IF释放达69.9±7.2%，抑制组胺（10⁻⁶ M）和五肽胃泌素（10⁻⁶ M）联合刺激的IF释放达64.2±9.1%。这是首次在体外证明生长抑素抑制IF释放。