Yang Yang, Tu Rui, Sun Wenji, Zhu Zhongliang, Zhang Yongmin
Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 710069, China; Biomedicine Key Laboratory of Shaanxi Province, College of Life Science, Northwest University, Xi'an 710069, China.
Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 710069, China; Institut Parisien de Chimie Moléculaire, CNRS UMR 8232, Université Pierre et Marie Curie-Paris 6,4 place Jussieu, 75005 Paris, France; Biomedicine Key Laboratory of Shaanxi Province, College of Life Science, Northwest University, Xi'an 710069, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Sep 15;1063:25-30. doi: 10.1016/j.jchromb.2017.08.013. Epub 2017 Aug 14.
Alantolactone and isoalantolactone isolated from many species of plants are a pair of positional isomers of CC bond. Previously, alantolactone and isoalantolactone have been proved to be good lead compounds for future anticancer agent development. Similarity of their molecular structures increases the separation difficulty for these two isomers on a conventional C column. Silver perchlorate (AgClO) as mobile phase additives with RP-HPLC for improving the separation was developed for rapid determination of the positional isomers in Inula racemosa Hook.f. The effects of the concentration of silver perchlorate on the separation of the analytes were investigated. The composition of acetonitrile and water containing 5.0% silver perchlorate in a 65:35 (v/v) ratio was used as mobile phase, in which they were well separated within a short period of time on the C column. The method was successfully applied to determine them in an extract of Inula racemosa Hook.f. root. Silver perchlorate in mobile phase can efficiently improve the separation of the positional isomers and could be applied to rapidly determinate their content in this plant.
从多种植物中分离得到的土木香内酯和异土木香内酯是一对碳碳键位置异构体。此前，已证明土木香内酯和异土木香内酯是未来抗癌药物开发的良好先导化合物。它们分子结构的相似性增加了这两种异构体在传统C柱上的分离难度。开发了以高氯酸银（AgClO）作为流动相添加剂的反相高效液相色谱法（RP-HPLC）用于改善分离效果，以快速测定总状土木香（Inula racemosa Hook.f.）中的位置异构体。研究了高氯酸银浓度对分析物分离的影响。以体积比65:35的含5.0%高氯酸银的乙腈和水的混合液作为流动相，在此条件下，它们在C柱上短时间内即可得到良好分离。该方法成功应用于测定总状土木香根提取物中的土木香内酯和异土木香内酯。流动相中的高氯酸银能够有效改善位置异构体的分离效果，可用于快速测定该植物中它们的含量。
