[反相高效液相色谱法同时测定款冬中三种倍半萜内酯]

[Simultaneous determination of three sesquiterpene lactones in Inula hupehensis by RP-HPLC].

作者信息

Wang Xiaolei, Li Xianghe, Guo Yi, Qi Jinlong, Su Linfei, Fu Yan

机构信息

Department of Drug Discovery, School of Pharmaceutical Science, Hebei Medical University, Shijiazhuang 050017, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2011 Sep;36(18):2520-4.

PMID:22256758

Abstract

OBJECTIVE

A RP-HPLC method was developed for simultaneous determination of bigelovin, ergolide and tomentosin in Inula hupehensis.

METHOD

An Agilent C18 column (4.6 mm x 250 mm, 5 microm) was used for separation at 40 degrees C. The mobile phase was acetonitrile-water, and the flow rate was 1.2 mL x min(-1). The detection wavelength was set at 210 nm.

RESULT

The method has good linearity in the ranges of 0.01792-0.1792 g x L(-1) (r =0.9999) for bigelovin, 0.0424-0.4240 g x L(-1) (r =0.9996) for ergolide, and 0.044 8-0.4480 g x L(-1) (r = 0.9996) for tomentosin. The average recoveries of bigelovin, ergolide, and tomentosin were 98.5%, 98.2%, 98.4%, with the RSD of 1. 3%, 1.3%, 1.7%, respectively. The results demonstrated that there was a significant difference in the contents of three sequterpene lactones among the tested Inulae Flos.

CONCLUSION

The results indicated that the present RP-HPLC method is simple, quick and accurate, and can be used for the quality control of I. hupehensis, especially for the authentication of Inulae Flos.

摘要

目的

建立一种反相高效液相色谱法（RP-HPLC）同时测定旋覆花中旋覆花素、麦角甾苷和绒毛旋覆花素的含量。

方法

采用安捷伦C18柱（4.6 mm×250 mm，5μm），于40℃下进行分离。流动相为乙腈-水，流速为1.2 mL·min⁻¹。检测波长设定为210 nm。

结果

该方法在旋覆花素0.01792 - 0.1792 g·L⁻¹（r = 0.9999）、麦角甾苷0.0424 - 0.4240 g·L⁻¹（r = 0.9996）、绒毛旋覆花素0.0448 - 0.4480 g·L⁻¹（r = 0.9996）范围内线性关系良好。旋覆花素、麦角甾苷和绒毛旋覆花素的平均回收率分别为98.5%、98.2%、98.4%，相对标准偏差（RSD）分别为1.3%、1.3%、1.7%。结果表明，所测旋覆花药材中三种倍半萜内酯含量存在显著差异。