Key Laboratory of Phytochemical R&D of Hunan Province and Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha, P. R. China.
College of Chemistry and Chemical Engineering, University of South China, Hengyang, P.R. China.
J Sep Sci. 2017 Nov;40(21):4128-4134. doi: 10.1002/jssc.201700487. Epub 2017 Sep 22.
Ginsenosides have been widely conceded as having various biological activities and are considered to be the active ingredient of ginseng. Nowadays, preparative high-performance liquid chromatography is considered to be a highly efficient method for ginseng saponins purification and preparation. However, in the process of practical application, due to the complex and varied composition of natural products and relatively simple pretreatment process, it is likely to block the chromatographic column and affect the separation efficiency and its service life. In this work, an orthogonal strategy was developed; in the first-dimension separation, reverse-phase macroporous resin was applied to remove impurities in ginseng crude extracts and classified ginseng extracts into protopanaxatriol and protopanaxadiol fractions. In the second-dimension separation, the obtained fractions were further separated by a preparative hydrophilic column, and finally yielded 11 pure compounds. Eight of them identified as ginsenoside Rh , Rg , Rd, Rc, Rb , Rb , Rg , and Re by standards comparison and electrospray ionization mass spectrometry. The purity of these ginsenosides was assessed by high-performance liquid chromatography with UV detection.
人参皂苷被广泛认为具有多种生物活性，被认为是人参的有效成分。如今，制备高效液相色谱法被认为是一种高效的人参皂苷纯化和制备方法。然而，在实际应用过程中，由于天然产物成分复杂多样，预处理过程相对简单，可能会堵塞色谱柱，影响分离效率及其使用寿命。在这项工作中，开发了一种正交策略；在一维分离中，反相大孔树脂用于去除人参粗提取物中的杂质，并将人参提取物分为原人参三醇和原人参二醇部分。在二维分离中，通过制备性亲水柱进一步分离得到的部分，最终得到 11 种纯化合物。其中 8 种通过与标准品比较和电喷雾电离质谱鉴定为人参皂苷 Rh、Rg、Rd、Rc、Rb、Rb、Rg 和 Re。这些人参皂苷的纯度通过高效液相色谱法用紫外检测进行评估。
