Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.
Fish Shellfish Immunol. 2017 Oct;69:227-235. doi: 10.1016/j.fsi.2017.08.023. Epub 2017 Aug 31.
MicroRNAs (miRNAs) play vital roles in modulating diverse metabolic processes in the liver, including lipid metabolism. Genetically improved farmed tilapia (GIFT, Oreochromis niloticus), an important aquaculture species in China, is susceptible to hepatic steatosis when reared in intensive culture systems. To investigate the miRNAs involved in GIFT lipid metabolism, two hepatic small RNA libraries from high-fat diet-fed and normal-fat diet-fed GIFT were constructed and sequenced using high-throughput sequencing technology. A total of 204 known and 56 novel miRNAs were identified by aligning the sequencing data with known Danio rerio miRNAs listed in miRBase 21.0. Six known miRNAs (miR-30a-5p, miR-34a, miR-145-5p, miR-29a, miR-205-5p, and miR-23a-3p) that were differentially expressed between the high-fat diet and normal-fat diet groups were validated by quantitative real-time PCR. Bioinformatics tools were used to predict the potential target genes of these differentially expressed miRNAs, and Gene Ontology enrichment analysis indicated that these miRNAs may play important roles in diet-induced hepatic steatosis in GIFT. Our results provide a foundation for further studies of the role of miRNAs in tilapia lipid homeostasis regulation, and may help to identify novel targets for therapeutic interventions to reduce the occurrence of fatty liver disease in farmed tilapia.
微小 RNA（miRNAs）在调节肝脏中的各种代谢过程中发挥着重要作用，包括脂质代谢。遗传改良型罗非鱼（GIFT，Oreochromis niloticus）是中国重要的水产养殖物种，在密集养殖系统中饲养时容易发生肝脂肪变性。为了研究与 GIFT 脂质代谢相关的 miRNAs，我们构建了两个来自高脂肪饮食喂养和正常脂肪饮食喂养 GIFT 的肝小 RNA 文库，并使用高通量测序技术对其进行了测序。通过将测序数据与 miRBase 21.0 中列出的已知 Danio rerio miRNAs 进行比对，共鉴定出 204 个已知和 56 个新的 miRNAs。通过定量实时 PCR 验证了在高脂肪饮食组和正常脂肪饮食组之间差异表达的 6 个已知 miRNAs（miR-30a-5p、miR-34a、miR-145-5p、miR-29a、miR-205-5p 和 miR-23a-3p）。使用生物信息学工具预测了这些差异表达 miRNAs 的潜在靶基因，GO 富集分析表明，这些 miRNAs 可能在 GIFT 饮食诱导的肝脂肪变性中发挥重要作用。我们的研究结果为进一步研究 miRNAs 在罗非鱼脂质稳态调节中的作用提供了基础，并可能有助于确定治疗干预减少养殖罗非鱼肝脂肪病发生的新靶标。
