MagQu Co., Ltd., Xindian District, New Taipei City, 231, Taiwan.
Department of Neurology, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, 100, Taiwan.
Sci Rep. 2017 Aug 24;7(1):9304. doi: 10.1038/s41598-017-09009-3.
Immunomagnetic reduction (IMR), which involves the use of antibody-functionalized magnetic nanoparticles to specifically label target biomarkers, was utilized to develop an assay for total tau protein in human plasma. The analytic properties of the IMR assay on tau protein were investigated. The limit of detection was found to be 0.026 pg/ml. Other properties such as Hook effect, assay linearity, dilution recovery range, reagent stability, interference test, and spiked recovery were also characterized. The ultra-sensitive IMR assay was applied to detect the plasma tau protein levels of subjects with prevalent neurodegenerative diseases, such as Alzheimer's disease (AD), mild cognitive impairment (MCI) due to AD, Parkinson's disease (PD), frontotemporal dementia (FTD) and vascular dementia (VD). The concentrations of plasma tau protein in patients with VD, PD, MCI due to AD, FTD, and AD patients were higher than that of healthy controls. Using an ROC curve analysis, the cutoff value for discriminating dementia patients from healthy controls was 17.43 pg/ml, resulting in 0.856 and 0.727 for clinical sensitivity and specificity, respectively. The area under the ROC curve was 0.908. These results imply that the IMR plasma tau assay would be useful to screen for prevalent neurodegenerative diseases.
免疫磁珠还原法（IMR）利用抗体功能化的磁性纳米颗粒特异性标记靶标生物标志物，用于开发人血浆总 tau 蛋白的测定方法。研究了 IMR 测定 tau 蛋白的分析特性。发现检测限为 0.026pg/ml。还对其他特性，如钩状效应、测定线性、稀释回收率范围、试剂稳定性、干扰试验和加标回收率进行了表征。超灵敏的 IMR 测定法用于检测常见神经退行性疾病（如阿尔茨海默病（AD）、AD 所致轻度认知障碍（MCI）、帕金森病（PD）、额颞叶痴呆（FTD）和血管性痴呆（VD））患者的血浆 tau 蛋白水平。VD、PD、AD 所致 MCI、FTD 患者的血浆 tau 蛋白浓度高于健康对照组。通过 ROC 曲线分析，区分痴呆患者和健康对照组的截断值为 17.43pg/ml，临床灵敏度和特异性分别为 0.856 和 0.727。ROC 曲线下面积为 0.908。这些结果表明，IMR 血浆 tau 测定法可用于筛查常见的神经退行性疾病。
