血小板因子 XIII 由钙蛋白酶激活。

Platelet factor XIII is activated by calpain.

作者信息

Ando Y, Imamura S, Yamagata Y, Kitahara A, Saji H, Murachi T, Kannagi R

出版信息

Biochem Biophys Res Commun. 1987 Apr 14;144(1):484-90. doi: 10.1016/s0006-291x(87)80535-1.

Abstract

The action of calpain (EC 3.4.22.17; Ca2+-dependent cysteine proteinase) on platelet factor XIII has been studied. Calpain I activated platelet factor XIII up to 76% of the maximum level observed with thrombin. Activation was accompanied by the limited proteolysis of the a subunit of platelet factor XIII to produce a 76 kDa fragment which was comparable to the proteolytic product by thrombin. Activation of platelet factor XIII by calpain was inhibited by EDTA, leupeptin, and endogenous calpain-specific inhibitor calpastatin. These findings suggest that calpain is responsible for the intracellular activation of platelet factor XIII.

摘要

已对钙蛋白酶（EC 3.4.22.17；钙依赖性半胱氨酸蛋白酶）对血小板因子 XIII 的作用进行了研究。钙蛋白酶 I 可将血小板因子 XIII 激活至凝血酶激活时所观察到的最大水平的 76%。激活过程伴随着血小板因子 XIII 的 a 亚基发生有限的蛋白水解，产生一个 76 kDa 的片段，该片段与凝血酶的蛋白水解产物相当。EDTA、亮抑酶肽和内源性钙蛋白酶特异性抑制剂钙蛋白酶抑制蛋白可抑制钙蛋白酶对血小板因子 XIII 的激活。这些发现表明，钙蛋白酶负责血小板因子 XIII 的细胞内激活。