Srinivasan A, York D, Ranganathan P, Ferguson R, Butler D, Feorino P, Kalyanaraman V, Jaffe H, Curran J, Anand R
Blood. 1987 Jun;69(6):1766-70.
The genetic diversity of the human immunodeficiency virus (HIV) isolated from transfusion-associated AIDS patients has been examined. Restriction enzyme mapping studies of integrated proviral DNA of donor and recipient origin demonstrated genomic variation between isolates. Analysis of the molecularly cloned viral genomes of one donor-recipient pair showed that virus from the recipient had restriction enzyme site differences from the donor, noticeably clustered in the env and orf-2 regions, and also had a greater number of restriction sites in common with the donor as well. These results suggest that HIV may undergo genomic variation in vivo. Comparison of donor-recipient viruses may further the understanding of the molecular basis for AIDS pathogenesis.
对从输血相关艾滋病患者中分离出的人类免疫缺陷病毒（HIV）的基因多样性进行了检测。对供体和受体来源的整合前病毒DNA进行的限制性酶切图谱研究表明，分离株之间存在基因组变异。对一对供体-受体的分子克隆病毒基因组分析显示，受体的病毒与供体的病毒在限制性酶切位点上存在差异，这些差异明显集中在env和orf-2区域，并且与供体也有更多共同的限制性位点。这些结果表明，HIV可能在体内发生基因组变异。比较供体-受体病毒可能会进一步加深对艾滋病发病机制分子基础的理解。
