Couto-Fernandez J C, Morgado M G, dos Santos J I, Galvão-Castro B
Laboratório Avançado de Saúde Pública, Centro de Pesquisas Gonçalo Moniz, Salvador, BA, Brasil.
Mem Inst Oswaldo Cruz. 1992 Apr-Jun;87(2):249-55. doi: 10.1590/s0074-02761992000200013.
To determine the genomic polymorphism and biological properties present in HIV-1 Brazilian isolates, we analyzed five viral isolates obtained from patients residing in Rio de Janeiro (P1 and P5), São Paulo (P3) and Bahia (P2 and P4) states. For each viral isolate in vitro characteristics such as replication rate, syncytium-inducing capacity and cell death were observed in lymphoblastoid (H9, CEM and peripheral blood mononuclear cells) as well as monocytoid (U937) cells. In addition, the evaluation of the restriction fragment length polymorphism of these isolates was also performed using a panel of endonucleases such as Hind III, Bgl II, Sac I, Pst I, Kpn I and Eco RI. One of the isolates (P1), showed the highest phenotypic and genotypic divergence, when compared to others. The results found suggest a HIV heterogeneity in Brazil similar to that already described in other regions of the world.
为了确定巴西HIV-1分离株中的基因组多态性和生物学特性，我们分析了从里约热内卢（P1和P5）、圣保罗（P3）和巴伊亚（P2和P4）州的患者中获得的5株病毒分离株。对于每株病毒分离株，在淋巴母细胞（H9、CEM和外周血单核细胞）以及单核细胞样（U937）细胞中观察了体外特性，如复制率、诱导合胞体能力和细胞死亡。此外，还使用一组核酸内切酶（如Hind III、Bgl II、Sac I、Pst I、Kpn I和Eco RI）对这些分离株的限制性片段长度多态性进行了评估。与其他分离株相比，其中一株分离株（P1）表现出最高的表型和基因型差异。研究结果表明，巴西的HIV存在异质性，这与世界其他地区已描述的情况类似。
