Robati Anaraki Mahmood, Mahboubi Shiva, Pirzadeh Tahereh, Lotfipour Farzaneh, Torkamanzad Naimeh
Dental and Periodontal Research Center, Tabriz University of Medical Science, Tabriz, Iran.
Department of Prosthetic Dentistry, Dental School, Tabriz University of Medical Science, Tabriz, Iran.
GMS Hyg Infect Control. 2017 Aug 8;12:Doc09. doi: 10.3205/dgkh000294. eCollection 2017.
The disinfection of dental stone casts using microwave radiation has been shown, but doubts remain regarding its efficacy under various conditions. The aim of the present study was to evaluate the efficacy of microwave disinfection on wet and dry dental stone casts contaminated by a resistant microorganism. In this in vitro study, 34 stone half-casts were prepared, contaminated with and divided into two groups. After drying the specimens of one group for 15 minutes using 450 W microwave energy, all the wet and dry specimens were exposed to 900 W microwave energy for 5 minutes. Specimens were then individually transferred to nutrient broth culture medium and after 10 minutes, one milliliter from each tube was cultured in nutrient agar media for 24 hours, and the colonies were counted in CFU/mL. Data were analyzed using multifactorial ANOVA and Bonferroni tests. Casts in both wet and dry groups were disinfected to a high level (6 log), with no statistically significant differences between them (P<0.05). According to the results, microwave irradiation can disinfect dental stone casts to a high degree, irrespective of moisture level. However, the result should be confirmed by exploring with other species of resistant microorganisms.
微波辐射用于消毒牙石膏模型已得到证实，但在各种条件下其有效性仍存在疑问。本研究的目的是评估微波消毒对受耐药微生物污染的湿、干牙石膏模型的效果。在这项体外研究中，制备了34个半牙石膏模型，用……污染并分为两组。使用450W微波能量将一组标本干燥15分钟后，所有湿、干标本均暴露于900W微波能量下5分钟。然后将标本分别转移至营养肉汤培养基中，10分钟后，从每个试管中取1毫升在营养琼脂培养基中培养24小时，并计算每毫升中的菌落形成单位（CFU/mL）。使用多因素方差分析和Bonferroni检验对数据进行分析。湿、干两组模型均被高效消毒（6个对数级），两组之间无统计学显著差异（P<0.05）。根据结果，无论湿度水平如何，微波辐射均可对牙石膏模型进行高度消毒。然而，该结果应通过对其他耐药微生物种类进行研究来证实。
