Yoshida M, Teramura M, Sakai M, Karasawa N, Nagatsu T, Nagatsu I
Brain Res. 1987 Apr 28;410(1):169-73. doi: 10.1016/s0006-8993(87)80041-0.
Antisera raised against glutamate or aspartate bound to bovine serum albumin were purified by affinity chromatography, and their specificities were verified by immunoblotting and by enzyme-linked immunosorbent assay. Immunohistochemical investigation using materials perfusion-fixed after long flushing demonstrated distinct laminar terminals with glutamate- or aspartate-like immunoreactivity throughout the limbic structures. This technique may offer a valuable tool for revealing the distribution of glutamatergic or aspartatergic nerve terminals.
通过亲和层析法纯化了针对与牛血清白蛋白结合的谷氨酸或天冬氨酸产生的抗血清，并通过免疫印迹和酶联免疫吸附测定法验证了它们的特异性。使用长时间冲洗后灌注固定的材料进行的免疫组织化学研究表明，在整个边缘结构中存在具有谷氨酸或天冬氨酸样免疫反应性的明显分层终末。这项技术可能为揭示谷氨酸能或天冬氨酸能神经终末的分布提供一种有价值的工具。
