Saito N, Kumoi K, Tanaka C
Neurosci Lett. 1986 Mar 28;65(1):89-93. doi: 10.1016/0304-3940(86)90125-4.
Antisera against aspartate (Asp) were prepared by immunizing rabbits with Asp conjugated to bovine serum albumin by glutaraldehyde, after which the antisera were purified using an Asp-immobilized epoxy-activated affinity column. The purified Asp antiserum showed no cross-reactivity, except for a 3% cross-reactivity against D-Asp. Asp-like immunoreactivity in mitral cells of the rat olfactory bulb was demonstrated, using this purified Asp antiserum.
通过用戊二醛将天冬氨酸（Asp）与牛血清白蛋白偶联后免疫兔子，制备了抗天冬氨酸（Asp）抗血清，然后使用固定有Asp的环氧活化亲和柱对该抗血清进行纯化。纯化后的Asp抗血清除了对D - Asp有3%的交叉反应性外，没有显示出交叉反应。使用这种纯化的Asp抗血清，在大鼠嗅球的二尖瓣细胞中证实了Asp样免疫反应性。
