Lundberg I, Högberg J, Kronevi T, Holmberg B
Cancer Lett. 1987 Jul;36(1):29-33. doi: 10.1016/0304-3835(87)90099-1.
Dioxane, perchloroethylene, and trichloroethylene were investigated as rat liver altered foci promoters in an initiation/promotion protocol. Animals were initiated with diethylnitrosamine, 30 mg/kg, injected i.p. 24 h after 2/3 partial hepatectomy. The chemical under study was administered by gavage once a day, 5 times a week for 7 weeks. Ten days after the last administration the animals were killed. Liver sections were stained for gamma-glutamyl-transpeptidase (GGT) and the number and total volume of GGT-positive foci was studied. Dioxane (1000 mg/kg) significantly increased the number and total volume of foci while a marginal effect was noted for the high dose of trichloroethylene (1100 mg/kg). A high dose of perchloroethylene (1100 mg/kg) had no effect.
在一项启动/促进实验方案中，对二氧六环、全氯乙烯和三氯乙烯作为大鼠肝脏癌前病变灶促进剂进行了研究。动物在2/3部分肝切除术后24小时腹腔注射30mg/kg的二乙基亚硝胺进行启动。所研究的化学物质通过灌胃给药，每周5次，每天1次，持续7周。最后一次给药10天后处死动物。对肝脏切片进行γ-谷氨酰转肽酶（GGT）染色，并研究GGT阳性灶的数量和总体积。二氧六环（1000mg/kg）显著增加了病灶的数量和总体积，而高剂量的三氯乙烯（1100mg/kg）有轻微影响。高剂量的全氯乙烯（1100mg/kg）没有效果。
