Pereira M A, Herren-Freund S L, Britt A L, Khoury M M
Cancer Lett. 1983 Sep;20(2):207-14. doi: 10.1016/0304-3835(83)90050-2.
The incidence of gamma-glutamyltranspeptidase (GGT)-positive foci induced by 0.3 mmol/kg diethylnitrosamine (DENA) followed by promotion with 500 ppm sodium phenobarbital in drinking water and was the same in Fischer 344, Sprague-Dawley and Wistar-Lewis rats. There was no difference in the level of GGT-foci initiated by DENA, 7,12-dimethylbenz[a]-anthracene (DMBA), or 1,2-dimethylhydrazine (DMH) followed by promotion with phenobarbital with respect to sex or route of administration including gavage and intraperitoneal injection. Maximal stimulation by partial hepatectomy of DENA initiation of GGT-foci occurred when the DENA was administered 18 h after the operation. Our results indicate that the optimal protocol for the rat liver foci assay consists of using partial hepatectomized rats of 1 of the 3 strains and of either sex. The test substance should be administered by either gavage or intraperitoneal injection so that maximal DNA binding coincides with the maximal rate of DNA replication resulting from partial hepatectomy.
给予0.3 mmol/kg二乙基亚硝胺（DENA），随后用500 ppm苯巴比妥钠饮水促癌，所诱导的γ-谷氨酰转肽酶（GGT）阳性灶发生率在Fischer 344、Sprague-Dawley和Wistar-Lewis大鼠中相同。DENA、7,12-二甲基苯并[a]蒽（DMBA）或1,2-二甲基肼（DMH）启动GGT阳性灶，随后用苯巴比妥促癌，无论性别或给药途径（包括灌胃和腹腔注射），GGT阳性灶水平均无差异。当在部分肝切除术后18小时给予DENA时，部分肝切除术对DENA启动GGT阳性灶的刺激作用最大。我们的结果表明，大鼠肝灶试验的最佳方案是使用3个品系中任一品系的部分肝切除大鼠，雌雄均可。受试物质应通过灌胃或腹腔注射给药，以使最大DNA结合与部分肝切除引起的最大DNA复制速率一致。
