Liu Juan, Ji Rui-Feng, Chen Tong, Yuan Yuan, Guo Juan, Wang Ying-Ping, Gao Wen-Yuan, Huang Lu-Qi
State Key Laboratory of Dao-di Herbs Breeding Base, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Institute of Special Animal and Plant, Chinese Academy of Agricultural Sciences, Changchun 130112, China.
Zhongguo Zhong Yao Za Zhi. 2017 Jul;42(13):2453-2459. doi: 10.19540/j.cnki.cjcmm.20170614.007.
The study is aimed to characterize the tissue expression of 10 key ginsenoside biosynthetic genes using bioinformatics method and real-time quantitative PCR. Heatmap and cluster analysis of 10 ginsenoside biosynthetic genes were performed in four-year-old Jilin ginseng. Using real-time quantitative PCR, the expression correlation of 10 key genes involved in ginsenoside biosynthesis was analyzed in different organs of four-year-old Jilin ginseng including, tissue culture seedling and adventitious root. Pearson correlation was used to analyze the relation between those 10 key genes involved in ginsenoside biosynthesis. The results showed that β-AS and CYP716A52v2 were expressed highly in root of Jilin ginseng and ginseng culture seedling, which was consistent with Ro distribution. In addition, CYP716A53v2 and CYP716A47 which involved in dammarane type ginsenoside biosynthesis were positively correlated, which revealed that the difference of ginsenoside distribution was caused by transport system.
本研究旨在利用生物信息学方法和实时定量PCR对10个关键人参皂苷生物合成基因的组织表达进行表征。对四年生吉林人参中的10个人参皂苷生物合成基因进行了热图和聚类分析。利用实时定量PCR，分析了四年生吉林人参不同器官（包括组织培养苗和不定根）中参与人参皂苷生物合成的10个关键基因的表达相关性。采用Pearson相关性分析人参皂苷生物合成中这10个关键基因之间的关系。结果表明，β-AS和CYP716A52v2在吉林人参根和人参培养苗中高表达，这与Ro分布一致。此外，参与达玛烷型人参皂苷生物合成的CYP716A53v2和CYP716A47呈正相关，这表明人参皂苷分布差异是由转运系统引起的。
