İbiş Osman, Kılıç Metin, Özcan Servet, Tez Coşkun
a Department of Agricultural Biotechnology, Faculty of Agriculture , Erciyes University , Kayseri , Turkey.
b Genome and Stem Cell Center, GENKOK, Erciyes University , Kayseri , Turkey.
Mitochondrial DNA A DNA Mapp Seq Anal. 2018 Aug;29(6):819-830. doi: 10.1080/24701394.2017.1365849. Epub 2017 Aug 25.
Although genetic diversity and phylogenetic status of the gray hamster (Cricetulus migratorius) have been investigated from different regions in previous studies, genetic data on this species from Turkey are still lacking, since previous data have been based on a limited number of gray hamsters sampled across the Anatolian part of Turkey. The aim of this study was to determine the genetic diversity of the Anatolian population and to reveal the phylogenetic relationships among the Anatolian population and conspecific populations of the gray hamster. The complete and partial fragments of mitochondrial Cyt b and 12S rRNA from the 20 Turkish samples were amplified and sequenced. Ten 12S rRNA (901 bp) and 15 Cyt b (1140 bp) haplotypes found in this work were not previously reported. Based on Bayesian, Maximum Likelihood, Neighbour-Joining and Median-Joining network analyses by using mitochondrial data under the name Cricetulus, the results of phylogenetic and network analyses indicated that there was a deep separation among the distinct lineages within the genus Cricetulus. When considering the position of the Turkish haplotypes in median joining network, the Anatolian part of Turkey may have hosted a source population of the gray hamster for expansion to adjacent regions in the past period. Additionally, the Anatolian population of gray hamster had relatively high haplotype diversity and the present study propounded the importance of data obtained from the Anatolian population of gray hamster to reveal the phylogenetic relationships among conspecific populations of the gray hamster.
尽管先前的研究已对不同地区的灰仓鼠（Cricetulus migratorius）的遗传多样性和系统发育地位进行了调查，但土耳其该物种的遗传数据仍然缺乏，因为先前的数据是基于在土耳其安纳托利亚地区采集的数量有限的灰仓鼠样本。本研究的目的是确定安纳托利亚种群的遗传多样性，并揭示安纳托利亚种群与灰仓鼠同种种群之间的系统发育关系。对来自20个土耳其样本的线粒体细胞色素b（Cyt b）和12S rRNA的完整和部分片段进行了扩增和测序。在这项研究中发现的10个12S rRNA（901 bp）和15个细胞色素b（1140 bp）单倍型以前未曾报道过。基于以Cricetulus命名的线粒体数据进行的贝叶斯分析、最大似然分析、邻接法分析和中介网络分析，系统发育和网络分析结果表明，Cricetulus属内不同谱系之间存在深度分化。考虑到土耳其单倍型在中介网络中的位置，土耳其的安纳托利亚地区在过去可能是灰仓鼠向邻近地区扩张的源种群所在地。此外，灰仓鼠的安纳托利亚种群具有相对较高的单倍型多样性，本研究提出了从灰仓鼠安纳托利亚种群获得的数据对于揭示灰仓鼠同种种群之间系统发育关系的重要性。
