Vun V F, Mahani M C, Lakim M, Ampeng A, Md-Zain B M
School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia.
Genet Mol Res. 2011 Mar 1;10(1):368-81. doi: 10.4238/vol10-1gmr1048.
Little is known about the classification and phylogenetic relationships of the leaf monkeys (Presbytis). We analyzed mitochondrial DNA sequences of cytochrome b (Cyt b) and 12S rRNA to determine the phylogenetic relationships of the genus Presbytis. Gene fragments of 388 and 371 bp of Cyt b and 12S rRNA, respectively, were sequenced from samples of Presbytis melalophos (subspecies femoralis, siamensis, robinsoni, and chrysomelas), P. rubicunda and P. hosei. The genus Trachypithecus (Cercopithecidae) was used as an outgroup. The Cyt b NJ and MP phylogeny trees showed P. m. chrysomelas to be the most primitive, followed by P. hosei, whereas 12S rRNA tree topology only indicated that these two species have close relationships with the other members of the genus. In our analysis, chrysomelas, previously classified as a subspecies of P. melalophos, was not included in either the P. m. femoralis clade or the P. m. siamensis clade. Whether or not there should be a separation at the species level remains to be clarified. The tree topologies also showed that P. m. siamensis is paraphyletic with P. m. robinsoni, and P. m. femoralis with P. rubicunda, in two different clades. Cyt b and 12S rRNA are good gene candidates for the study of phylogenetic relationships at the species level. However, the systematic relationships of some subspecies in this genus remain unclear.
关于叶猴属（Presbytis）的分类及系统发育关系，人们了解甚少。我们分析了细胞色素b（Cyt b）和12S核糖体RNA的线粒体DNA序列，以确定叶猴属的系统发育关系。分别从黑叶猴（Presbytis melalophos，亚种包括股斑叶猴、暹罗叶猴、罗宾逊叶猴和金背叶猴）、赤叶猴（P. rubicunda）和何氏叶猴（P. hosei）的样本中，对Cyt b的388 bp基因片段和12S核糖体RNA的371 bp基因片段进行了测序。豚尾叶猴属（Trachypithecus，猕猴科）被用作外类群。Cyt b的邻接法（NJ）和最大简约法（MP）系统发育树显示，金背叶猴是最原始的，其次是何氏叶猴，而12S核糖体RNA树的拓扑结构仅表明这两个物种与该属的其他成员关系密切。在我们的分析中，先前被归类为黑叶猴一个亚种的金背叶猴，既不包含在股斑叶猴分支中，也不包含在暹罗叶猴分支中。是否应该在物种层面进行划分仍有待阐明。树的拓扑结构还显示，在两个不同的分支中，暹罗叶猴与罗宾逊叶猴、股斑叶猴与赤叶猴均形成并系群。Cyt b和12S核糖体RNA是研究物种水平系统发育关系的良好基因候选对象。然而，该属中一些亚种的系统关系仍不明确。
