Vilia Maria Giovanna, Tocchetti Marta, Fonte Eleonora, Sana Ilenia, Muzio Marta
Cell signaling unit, division of experimental oncology, IRCCS San Raffaele hospital, Milano, Italy.
Eur Cytokine Netw. 2017 Jun 1;28(2):63-69. doi: 10.1684/ecn.2017.0395.
IL-1R8, also known as SIGIRR or TIR8, is a trans-membrane protein belonging to the IL-1 receptor family. The human gene includes ten exons, and alternative splicing can result in different isoforms. We, herein, characterized a longer isoform of IL-1R8 containing an in-frame additional sequence between the TIR domain and the C-terminal portion of the protein. IL-1R8 Long (IL-1R8L1) mRNA was specifically expressed and regulated in distinct cell lines, in a manner similar to the classic isoform. Overexpression of IL-1R8L1 resulted in the production of a corresponding protein that showed a pattern of cell localization similar to the classic isoform. An antibody directed against an IL-1R8L1 specific peptide, detected this novel isoform in different cell lines and tissues where this protein may complement the anti-inflammatory functions of classic IL-1R8.
白细胞介素-1受体8（IL-1R8），也称为单免疫球蛋白白细胞介素-1受体相关分子（SIGIRR）或TIR8，是一种属于白细胞介素-1受体家族的跨膜蛋白。人类基因包含十个外显子，可变剪接可产生不同的异构体。在此，我们鉴定了一种较长的IL-1R8异构体，其在TIR结构域与蛋白质的C末端部分之间含有一个框内附加序列。白细胞介素-1受体8长链（IL-1R8L1）信使核糖核酸（mRNA）在不同细胞系中特异性表达并受到调控，其方式与经典异构体相似。IL-1R8L1的过表达导致产生一种相应的蛋白质，该蛋白质显示出与经典异构体相似的细胞定位模式。一种针对IL-1R8L1特异性肽的抗体，在不同细胞系和组织中检测到了这种新型异构体，在此该蛋白质可能补充经典IL-1R8的抗炎功能。
