• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

3-苯乙酸-5,15-二乙酸瑞香毒素的结构鉴定及体外抗癌活性

Structure Identification and In Vitro Anticancer Activity of Lathyrol-3-phenylacetate-5,15-diacetate.

作者信息

Zhang Jian-Ye, Huang Wen-Jing, Sun Hong-Mei, Liu Yun, Zhao Xiao-Qin, Tang Si-Li, Sun Ming-Na, Wang Sheng, Li Jia-Jun, Zhang Ling-Ling, Zhou Jun-Hua, Pan Qian-Rong, Chen Hu-Biao

机构信息

Key Laboratory of Molecular and Clinical Pharmacology, School of Pharmaceutical Sciences and the Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou 511436, China.

Infinitus (China) Company Ltd., Jiangmen 529156, China.

出版信息

Molecules. 2017 Aug 25;22(9):1412. doi: 10.3390/molecules22091412.

DOI:10.3390/molecules22091412
PMID:28841191
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6151716/
Abstract

Natural products from the genus show attention-attracting activities, such as anticancer activity. In this article, classical isolation and structure identification were used in a study on Caper Euphorbia Seed. Subsequently, MTT and wound healing assays, flow cytometry, western blotting, Hoechst 33258 staining and fluorescence microscopy examination were applied to investigate the anticancer activity of the obtained compounds. In a result, lathyrol-3-phenyl- acetate-5,15-diacetate (deoxy Euphorbia factor L1, DEFL1) was isolated from Caper Euphorbia Seed. Moreover, the NMR signals were totally assigned. DEFL1 showed potent inhibition against lung cancer A549 cells, with an IC value of 17.51 ± 0.85 μM. Furthermore, DEFL1 suppressed wound healing of A549 cells in a concentration-dependent manner. Mechanically, DEFL1 induced apoptosis, with involvement of an increase of reactive oxygen species (ROS), decrease of mitochondrial membrane potential (ΔΨm), release of cytochrome , activity raise of caspase-9 and 3. Characteristic features of apoptosis were observed by fluorescence microscopy. In summary, DEFL1 inhibited growth and induced apoptosis in lung cancer A549 cells via a mitochondrial pathway.

摘要

大戟属的天然产物具有吸引注意力的活性，如抗癌活性。在本文中，采用经典的分离和结构鉴定方法对续随子种子进行了研究。随后，运用MTT和伤口愈合试验、流式细胞术、蛋白质免疫印迹法、Hoechst 33258染色和荧光显微镜检查来研究所得化合物的抗癌活性。结果从续随子种子中分离出了瑞香烷型二萜酯类化合物lathyrol-3-phenyl- acetate-5,15-diacetate（去氧大戟因子L1，DEFL1）。此外，对其核磁共振信号进行了全归属。DEFL1对肺癌A549细胞显示出强效抑制作用，IC值为17.51±0.85μM。此外，DEFL1以浓度依赖的方式抑制A549细胞的伤口愈合。机制上，DEFL1诱导细胞凋亡，涉及活性氧（ROS）增加、线粒体膜电位（ΔΨm）降低、细胞色素c释放、半胱天冬酶-9和3活性升高。通过荧光显微镜观察到了凋亡的特征。综上所述，DEFL1通过线粒体途径抑制肺癌A549细胞的生长并诱导其凋亡

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c14e/6151716/e105fd6b7b79/molecules-22-01412-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c14e/6151716/10348bf9f795/molecules-22-01412-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c14e/6151716/c8193e4dcdce/molecules-22-01412-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c14e/6151716/488227788c82/molecules-22-01412-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c14e/6151716/b273fa90669f/molecules-22-01412-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c14e/6151716/3be326ebd170/molecules-22-01412-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c14e/6151716/9365c89782d4/molecules-22-01412-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c14e/6151716/e105fd6b7b79/molecules-22-01412-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c14e/6151716/10348bf9f795/molecules-22-01412-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c14e/6151716/c8193e4dcdce/molecules-22-01412-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c14e/6151716/488227788c82/molecules-22-01412-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c14e/6151716/b273fa90669f/molecules-22-01412-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c14e/6151716/3be326ebd170/molecules-22-01412-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c14e/6151716/9365c89782d4/molecules-22-01412-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c14e/6151716/e105fd6b7b79/molecules-22-01412-g007.jpg

相似文献

1
Structure Identification and In Vitro Anticancer Activity of Lathyrol-3-phenylacetate-5,15-diacetate.3-苯乙酸-5,15-二乙酸瑞香毒素的结构鉴定及体外抗癌活性
Molecules. 2017 Aug 25;22(9):1412. doi: 10.3390/molecules22091412.
2
Evaluation of the cytotoxicity, cell-cycle arrest, and apoptotic induction by Euphorbia hirta in MCF-7 breast cancer cells.大戟草对MCF-7乳腺癌细胞的细胞毒性、细胞周期阻滞及凋亡诱导作用的评估。
Pharm Biol. 2016 Jul;54(7):1223-36. doi: 10.3109/13880209.2015.1064451. Epub 2015 Jul 8.
3
The Euphorbia lunulata Bge extract inhibits proliferation of human hepatoma HepG2 cells and induces apoptosis.地锦草提取物抑制人肝癌HepG2细胞增殖并诱导其凋亡。
J BUON. 2013 Apr-Jun;18(2):491-5.
4
Structure identification of Euphorbia factor L3 and its induction of apoptosis through the mitochondrial pathway.冬凌草甲素 L3 的结构鉴定及其通过线粒体途径诱导细胞凋亡。
Molecules. 2011 Apr 15;16(4):3222-31. doi: 10.3390/molecules16043222.
5
Genistein induced anticancer effects on pancreatic cancer cell lines involves mitochondrial apoptosis, G/Gcell cycle arrest and regulation of STAT3 signalling pathway.染料木黄酮对胰腺癌细胞系的抗癌作用涉及线粒体凋亡、G/G 细胞周期阻滞和 STAT3 信号通路的调节。
Phytomedicine. 2018 Jan 15;39:10-16. doi: 10.1016/j.phymed.2017.12.001. Epub 2017 Dec 5.
6
New 6(17)-epoxylathyrane diterpene: aellinane from Euphorbia aellenii induces apoptosis via mitochondrial pathway in ovarian cancer cell line.新型6(17)-环氧千金二萜：来自埃氏大戟的埃里烷通过线粒体途径诱导卵巢癌细胞系凋亡。
Toxicol Mech Methods. 2017 Oct;27(8):622-630. doi: 10.1080/15376516.2017.1347735. Epub 2017 Jul 28.
7
Elephantopus scaber induces apoptosis through ROS-dependent mitochondrial signaling pathway in HCT116 human colorectal carcinoma cells.地胆草通过活性氧依赖的线粒体信号通路诱导HCT116人结肠癌细胞凋亡。
J Ethnopharmacol. 2015 Jun 20;168:291-304. doi: 10.1016/j.jep.2015.03.072. Epub 2015 Apr 8.
8
Euphorbia factor L2 induces apoptosis in A549 cells through the mitochondrial pathway.大戟因子L2通过线粒体途径诱导A549细胞凋亡。
Acta Pharm Sin B. 2017 Jan;7(1):59-64. doi: 10.1016/j.apsb.2016.06.008. Epub 2016 Jul 30.
9
β-elemene reverses the drug resistance of lung cancer A549/DDP cells via the mitochondrial apoptosis pathway.β-榄香烯通过线粒体凋亡途径逆转肺癌A549/DDP细胞的耐药性。
Oncol Rep. 2014 May;31(5):2131-8. doi: 10.3892/or.2014.3083. Epub 2014 Mar 12.
10
Jatropha-6(17),11E-diene class derivatives induce apoptosis effects in OVCAR-3 and Caov-4 ovarian cancer cell lines via a mitochondrial pathway.麻风树-6(17),11E-二烯类衍生物通过线粒体途径诱导OVCAR-3和Caov-4卵巢癌细胞系的凋亡效应。
Biochem Cell Biol. 2017 Dec;95(6):616-627. doi: 10.1139/bcb-2017-0034. Epub 2017 Jun 27.

引用本文的文献

1
Unveiling the anticancer potential of (L.) Wight& Arn.: Evidences from cytotoxicity studies, apoptosis analysis, and molecular docking.揭示（L.）Wight&Arn.的抗癌潜力：来自细胞毒性研究、凋亡分析和分子对接的证据。
3 Biotech. 2024 Oct;14(10):245. doi: 10.1007/s13205-024-04096-2. Epub 2024 Sep 25.
2
Targeting SERCA2 in Anti-Tumor Drug Discovery.在抗肿瘤药物研发中靶向肌浆网/内质网钙ATP酶2（SERCA2）
Curr Drug Targets. 2025;26(1):1-16. doi: 10.2174/0113894501325497240918042654.
3
Diversely functionalized isoquinolines and their core-embedded heterocyclic frameworks: a privileged scaffold for medicinal chemistry.

本文引用的文献

1
Parthenolide suppresses non-small cell lung cancer GLC-82 cells growth via B-Raf/MAPK/Erk pathway.小白菊内酯通过B-Raf/MAPK/Erk信号通路抑制非小细胞肺癌GLC-82细胞的生长。
Oncotarget. 2017 Apr 4;8(14):23436-23447. doi: 10.18632/oncotarget.15584.
2
Euphorbia factor L2 induces apoptosis in A549 cells through the mitochondrial pathway.大戟因子L2通过线粒体途径诱导A549细胞凋亡。
Acta Pharm Sin B. 2017 Jan;7(1):59-64. doi: 10.1016/j.apsb.2016.06.008. Epub 2016 Jul 30.
3
MicroRNA-302b targets Mcl-1 and inhibits cell proliferation and induces apoptosis in malignant pleural mesothelioma cells.
多样化功能化异喹啉及其核心嵌入的杂环骨架：药物化学的优势骨架
RSC Med Chem. 2023 Aug 25;14(12):2509-2534. doi: 10.1039/d3md00248a. eCollection 2023 Dec 13.
4
Effect of lathyrol derivatives on non-small cell lung cancer and the possible mechanism.拉蒂洛尔衍生物对非小细胞肺癌的作用及可能机制。
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2022 Feb 28;47(2):143-152. doi: 10.11817/j.issn.1672-7347.2022.210104.
5
The targeting of non‑coding RNAs by curcumin: Facts and hopes for cancer therapy (Review).姜黄素靶向非编码 RNA：癌症治疗的事实和希望（综述）。
Oncol Rep. 2019 Jul;42(1):20-34. doi: 10.3892/or.2019.7148. Epub 2019 May 3.
6
Integrated Bioinformatics Analysis the Function of RNA Binding Proteins (RBPs) and Their Prognostic Value in Breast Cancer.整合生物信息学分析RNA结合蛋白（RBPs）在乳腺癌中的功能及其预后价值。
Front Pharmacol. 2019 Mar 1;10:140. doi: 10.3389/fphar.2019.00140. eCollection 2019.
7
A Natural Isoquinoline Alkaloid With Antitumor Activity: Studies of the Biological Activities of Berberine.一种具有抗肿瘤活性的天然异喹啉生物碱：黄连素的生物活性研究。
Front Pharmacol. 2019 Feb 14;10:9. doi: 10.3389/fphar.2019.00009. eCollection 2019.
8
Special Issue: Natural Products: Anticancer and Beyond.特刊：天然产物：抗癌及其他作用
Molecules. 2018 May 23;23(6):1246. doi: 10.3390/molecules23061246.
微小RNA-302b靶向髓细胞白血病-1蛋白，抑制恶性胸膜间皮瘤细胞的增殖并诱导其凋亡。
Am J Cancer Res. 2016 Sep 1;6(9):1996-2009. eCollection 2016.
4
Curcumin inhibits cell growth and invasion and induces apoptosis through down-regulation of Skp2 in pancreatic cancer cells.姜黄素通过下调胰腺癌细胞中Skp2的表达来抑制细胞生长和侵袭，并诱导细胞凋亡。
Am J Cancer Res. 2016 Sep 1;6(9):1949-1962. eCollection 2016.
5
Afatinib down-regulates MCL-1 expression through the PERK-eIF2α-ATF4 axis and leads to apoptosis in head and neck squamous cell carcinoma.阿法替尼通过PERK-eIF2α-ATF4轴下调MCL-1表达，并导致头颈部鳞状细胞癌发生凋亡。
Am J Cancer Res. 2016 Aug 1;6(8):1708-19. eCollection 2016.
6
MiR-153 promotes breast cancer cell apoptosis by targeting HECTD3.微小RNA-153通过靶向HECTD3促进乳腺癌细胞凋亡。
Am J Cancer Res. 2016 Jul 1;6(7):1563-71. eCollection 2016.
7
Synthesis of a novel adamantyl nitroxide derivative with potent anti-hepatoma activity in vitro and in vivo.一种新型金刚烷基氮氧化物衍生物的合成及其在体外和体内的高效抗肝癌活性
Am J Cancer Res. 2016 Jun 1;6(6):1271-86. eCollection 2016.
8
Aldehyde dehydrogenase inhibition combined with phenformin treatment reversed NSCLC through ATP depletion.乙醛脱氢酶抑制与苯乙双胍联合治疗通过消耗ATP逆转非小细胞肺癌。
Oncotarget. 2016 Aug 2;7(31):49397-49410. doi: 10.18632/oncotarget.10354.
9
An Ingenol Derived from Euphorbia kansui Induces Hepatocyte Cytotoxicity by Triggering G0/G1 Cell Cycle Arrest and Regulating the Mitochondrial Apoptosis Pathway in Vitro.源于甘遂的大戟醇酯通过触发G0/G1期细胞周期阻滞和调控体外线粒体凋亡途径诱导肝细胞毒性。
Molecules. 2016 Jun 22;21(6):813. doi: 10.3390/molecules21060813.
10
Two Trichothecene Mycotoxins from Myrothecium roridum Induce Apoptosis of HepG-2 Cells via Caspase Activation and Disruption of Mitochondrial Membrane Potential.来自漆斑菌的两种单端孢霉烯族真菌毒素通过半胱天冬酶激活和线粒体膜电位破坏诱导肝癌细胞系HepG-2细胞凋亡。
Molecules. 2016 Jun 17;21(6):781. doi: 10.3390/molecules21060781.