Gao Feng, Fu Zhaoying, Tian Hongying, He Zhiying
Department of Clinical Medicine, School of Medicine, Yan'an University, Yan'an, Shaanxi Province, Yan'an, China.
J BUON. 2013 Apr-Jun;18(2):491-5.
The purpose of this study was to determine the effect of Euphorbia lunulata Bge extract on the proliferation of human hepatoma HepG2 cell line.
Different dilutions of Euphorbia lunulata Bge extract were used to treat human hepatoma HepG2 cells. Hoechst 33258 and PI-staining fluorescence microscopy were utilized to observe the nuclear morphological changes of apoptotic cells. Flow cytometry was used to detect the rates of apoptosis and apoptotic peaks. Western blotting was performed to analyze the subcellular distribution of cytochrome C.
The Euphorbia lunulata Bge extract was found to inhibit the proliferation of human hepatoma HepG2 cells via a time and concentration-dependent manner.
Altogether, the results suggest that the Euphorbia lunulata Bge extract is effective in inhibiting the proliferation of human hepatoma HepG2 cells and inducing cell apoptosis. The mechanism may be related to the mitochondrial pathways or cellular apoptosis pathways.
本研究旨在确定月腺大戟提取物对人肝癌HepG2细胞系增殖的影响。
用不同稀释度的月腺大戟提取物处理人肝癌HepG2细胞。采用Hoechst 33258和PI染色荧光显微镜观察凋亡细胞核形态变化。用流式细胞术检测凋亡率和凋亡峰。采用蛋白质免疫印迹法分析细胞色素C的亚细胞分布。
发现月腺大戟提取物能通过时间和浓度依赖性方式抑制人肝癌HepG2细胞的增殖。
总之，结果表明月腺大戟提取物能有效抑制人肝癌HepG2细胞的增殖并诱导细胞凋亡。其机制可能与线粒体途径或细胞凋亡途径有关。
