Beijing National Laboratory for Molecular Sciences, CAS Key Lab of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, Zhonguancun, Beijing, 100190, China.
School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, Beijing, 100049, China.
Macromol Rapid Commun. 2017 Oct;38(20). doi: 10.1002/marc.201700408. Epub 2017 Aug 25.
Different from the conventional irreversible covalent conjugations, a simple and efficient dynamic Schiff base covalent assembly is developed to construct the stable and smart dipeptide-protein hydrogels under mild conditions. Diphenylalanine-hemoglobin hydrogel is chosen to investigate the gelation formation process and mechanism. It is found that such assembled dipeptide-protein hydrogels are sensitive to pH variation, and simultaneously the proteins can be released without changing the native secondary structures from the gels. Furthermore, these adaptive hydrogels can encapsulate a series of small molecules, multicomponent proteins, and functional nanoparticles. These versatile hydrogels may find a great potential in bioapplications.
与传统的不可逆共价键合不同，本研究开发了一种简单高效的动态席夫碱共价组装方法，可在温和条件下构建稳定的智能二肽-蛋白质水凝胶。选择二苯丙氨酸-血红蛋白水凝胶来研究凝胶形成过程和机制。结果发现，这种组装的二肽-蛋白质水凝胶对 pH 值的变化敏感，同时蛋白质可以在不改变凝胶中原有的二级结构的情况下从凝胶中释放出来。此外，这些适应性水凝胶可以封装一系列小分子、多种蛋白质和功能纳米粒子。这些多功能水凝胶在生物应用中可能具有巨大的潜力。
